Methods to prepare proteins that include a specific modification at a desired position are
essential for understanding their cellular functions and physical properties in living systems …

The covalent attachment of Ub (ubiquitin) to target proteins (ubiquitylation) represents one of
the most versatile PTMs (post-translational modifications) in eukaryotic cells. Substrate …

Activity‐based E2 conjugating enzyme (E2)‐ubiquitin (Ub) probes have recently emerged as
effective tools for studying the molecular mechanism of E3 ligase (E3)‐catalyzed …

Abstract Poly (ADP-ribosyl) ation (PARylation) is a multifaceted post-translational
modification, carried out by poly (ADP-ribosyl) transferases (poly-ARTs, PARPs), which play …

Deubiquitinating enzymes are key regulators in the ubiquitin system and an emerging class
of drug targets. These proteases disassemble polyubiquitin chains and many …

Chemical ubiquitination is an effective approach for accessing structurally defined, atypical
ubiquitin (Ub) chains that are difficult to prepare by other techniques. Herein, we describe a …

Ubiquitination regulates, via different modes of modifications, a variety of biological
processes, and aberrations in the process have been implicated in the pathogenesis of …

Conspectus Chemical protein synthesis has been proved as an efficient way to afford
medium-sized proteins with high homogeneity in workable quantities for various …

S-to-N acyl transfer is a high-yielding chemoselective process for amide bond formation. It is
widely utilized by chemists for synthetic applications, including peptide and protein …

A promising approach in cancer therapy is to find ligands that directly bind ubiquitin (Ub)
chains. However, finding molecules capable of tightly and specifically binding Ub chains is …