The investigation of biological processes by chemical methods, commonly referred to as
chemical biology, often requires chemical access to biologically relevant macromolecules …

▪ Abstract Expressed protein ligation (EPL) is a protein engineering approach that allows
recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined …

The matrix metalloproteinases (MMP)-2 and-9, also known as the gelatinases have been
long recognized as major contributors to the proteolytic degradation of extracellular matrix …

▪ Abstract Protein splicing is a form of posttranslational processing that consists of the
excision of an intervening polypeptide sequence, the intein, from a protein, accompanied by …

Biophysical techniques such as fluorescence spectroscopy and nuclear magnetic
resonance (NMR) spectroscopy provide a window into the inner workings of proteins. These …

Antimicrobial peptides (AMPs) constitute an important component of the innate immune
system in a variety of organisms. Buforin I is a 39-amino acid AMP that was first isolated from …

▪ Abstract Inteins are genetic elements that disrupt the coding sequence of genes. However,
in contrast to introns, inteins are transcribed and translated together with their host protein …

Protein splicing is a posttranslational process in which an intein segment excises itself from
two flanking peptides, referred to as exteins. In the native context, protein splicing results in …

Inteins excise themselves out of precursor proteins by the protein splicing reaction and have
emerged as valuable protein engineering tools in numerous and diverse biotechnological …

The discovery of inteins in the early 1990s opened the door to a wide variety of new
technologies. Early engineered inteins from various sources allowed the development of self …