Finding faster and simpler ways to screen protein sequence space to enable the
identification of new biocatalysts for asymmetric synthesis remains both a challenge and a …

Deoxy-d-ribose-5-phosphate aldolase (DERA) is a class I aldolase that offers access to
several building blocks for organic synthesis. It catalyzes the stereoselective C–C bond …

Abstract The Entner–Doudoroff (ED) pathway provides an alternative to glycolysis. It
converts 6-phosphogluconate (6-PG) to glyceraldehyde-3-phosphate and pyruvate in two …

Abstract Deoxyribose-5-phosphate aldolases (DERAs, EC 4.1. 2.4) are acetaldehyde-
dependent, Class I aldolases catalyzing in nature a reversible aldol reaction between an …

A cell-free enantioselective transformation of the carbon atom of CO2 has never been
reported. In the urgent context of transforming CO2 into products of high value, the …

The preparation of α-functionalized organic acids can be greatly simplified by adopting a
protocol involving the catalytic assembly of achiral building blocks. However, the enzymatic …

Class I aldolases catalyze asymmetric aldol addition reactions and have found extensive
application in the biocatalytic synthesis of chiral β-hydroxy-carbonyl compounds. However …

Abstract In this work, deoxyribose-5-phosphate aldolase (Ec DERA, EC 4.1. 2.4) from
Escherichia coli was chosen as the protein engineering target for improving the substrate …

The congested nature of quaternary carbons hinders their preparation, most notably when
stereocontrol is required. Here we report a biocatalytic method for the creation of quaternary …

Controlling regio-and stereoselectivity of aldol additions is generally challenging. Here we
show that an artificial aldolase with high specificity for acetone as the aldol donor can be …