Advances and challenges in metatranscriptomic analysis

M Shakya, CC Lo, PSG Chain - Frontiers in genetics, 2019 - frontiersin.org
Sequencing-based analyses of microbiomes have traditionally focused on addressing the
question of community membership and profiling taxonomic abundance through amplicon …

Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome

VJ Carrión, J Perez-Jaramillo, V Cordovez, V Tracanna… - Science, 2019 - science.org
Microorganisms living inside plants can promote plant growth and health, but their genomic
and functional diversity remain largely elusive. Here, metagenomics and network inference …

Tax4Fun2: prediction of habitat-specific functional profiles and functional redundancy based on 16S rRNA gene sequences

F Wemheuer, JA Taylor, R Daniel, E Johnston… - Environmental …, 2020 - Springer
Abstract Background Sequencing of 16S rRNA genes has become a powerful technique to
study microbial communities and their responses towards changing environmental …

Tax4Fun: predicting functional profiles from metagenomic 16S rRNA data

KP Aßhauer, B Wemheuer, R Daniel… - Bioinformatics, 2015 - academic.oup.com
Motivation: The characterization of phylogenetic and functional diversity is a key element in
the analysis of microbial communities. Amplicon-based sequencing of marker genes, such …

Stable core virome despite variable microbiome after fecal transfer

F Broecker, G Russo, J Klumpp, K Moelling - Gut Microbes, 2017 - Taylor & Francis
ABSTRACT We recently described the 4.5-year time course of the enteric bacterial
microbiota and virome of a patient cured from recurrent Clostridium difficile infection (rCDI) …

Wastewater effluents cause microbial community shifts and change trophic status

JE Ruprecht, SC Birrer, KA Dafforn, SM Mitrovic… - Water research, 2021 - Elsevier
Widespread wastewater pollution is one of the greatest challenges threatening the
sustainable management of rivers globally. Understanding microbial responses to gradients …

Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing

SST Mak, S Gopalakrishnan, C Carøe, C Geng… - …, 2017 - academic.oup.com
Ancient DNA research has been revolutionized following development of next-generation
sequencing platforms. Although a number of such platforms have been applied to ancient …

The active microbial community more accurately reflects the anaerobic digestion process: 16S rRNA (gene) sequencing as a predictive tool

J De Vrieze, AJ Pinto, WT Sloan, UZ Ijaz - Microbiome, 2018 - Springer
Background Amplicon sequencing methods targeting the 16S rRNA gene have been used
extensively to investigate microbial community composition and dynamics in anaerobic …

Statin therapy causes gut dysbiosis in mice through a PXR-dependent mechanism

JA Caparrós-Martín, RR Lareu, JP Ramsay, J Peplies… - Microbiome, 2017 - Springer
Background Statins are a class of therapeutics used to regulate serum cholesterol and
reduce the risk of heart disease. Although statins are highly effective in removing cholesterol …

MOCAT2: a metagenomic assembly, annotation and profiling framework

JR Kultima, LP Coelho, K Forslund… - …, 2016 - academic.oup.com
MOCAT2 is a software pipeline for metagenomic sequence assembly and gene prediction
with novel features for taxonomic and functional abundance profiling. The automated …