[HTML][HTML] Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
TM Khlebodarova, NV Bogacheva, AV Zadorozhny… - Microorganisms, 2024 - mdpi.com
In the 1980s, Escherichia coli was the preferred host for heterologous protein expression
owing to its capacity for rapid growth in complex media; well-studied genetics; rapid and …
owing to its capacity for rapid growth in complex media; well-studied genetics; rapid and …
[HTML][HTML] Kinetic and thermodynamic studies of a novel acid protease from Aspergillus foetidus
PM Souza, B Aliakbarian, EX Ferreira Filho… - International Journal of …, 2015 - Elsevier
The kinetics of a thermostable extracellular acid protease produced by an Aspergillus
foetidus strain was investigated at different pH, temperatures and substrate concentrations …
foetidus strain was investigated at different pH, temperatures and substrate concentrations …
Biochemical, thermodynamic and structural characteristics of a biotechnologically compatible alkaline protease from a haloalkaliphilic, Nocardiopsis dassonvillei OK …
This report describes purification strategies, biochemical properties and thermodynamic
analysis of an alkaline serine protease from a marine actinomycete, Nocardiopsis …
analysis of an alkaline serine protease from a marine actinomycete, Nocardiopsis …
[HTML][HTML] Thermodynamic investigation of an alkaline protease from Aspergillus tamarii URM4634: a comparative approach between crude extract and purified enzyme
OS da Silva, RL de Oliveira, J de Carvalho Silva… - International journal of …, 2018 - Elsevier
The thermostable crude proteolytic extract and purified protease produced by Aspergillus
tamarii URM4634 were investigated at different temperatures. The activity results were used …
tamarii URM4634 were investigated at different temperatures. The activity results were used …
[HTML][HTML] Temperature and pH Optimization for Protease Production Fermented by Yarrowia lipolytica from Agro-Industrial Waste
J López-Trujillo, M Mellado-Bosque… - Fermentation, 2023 - mdpi.com
The use of yeasts for the production of proteases has increased in demand in recent years.
Y. lipolytica has been reported as a strain with high yields of protease production. This work …
Y. lipolytica has been reported as a strain with high yields of protease production. This work …
Catalytic, kinetic and thermodynamic properties of stabilized Bacillus stearothermophilus alkaline protease
MA Abdel-Naby, SA Ahmed, HR Wehaidy… - International journal of …, 2017 - Elsevier
Bacillus stearothermophilus alkaline protease was conjugated to several oxidized
polysaccharides of different chemical structure. The conjugates were evaluated for the …
polysaccharides of different chemical structure. The conjugates were evaluated for the …
Two steps purification, biochemical characterization, thermodynamics and structure elucidation of thermostable alkaline serine protease from Nocardiopsis alba strain …
JV Chauhan, RP Mathukiya, SP Singh… - International Journal of …, 2021 - Elsevier
The Nocardiopsis alba strain OM-5 showed maximum protease production in submerged
culture. The OM-5 protease was purified by hydrophobic interaction chromatography. The …
culture. The OM-5 protease was purified by hydrophobic interaction chromatography. The …
Evaluating the industrial potential of naturally occurring proteases: A focus on kinetic and thermodynamic parameters
M Salehi - International Journal of Biological Macromolecules, 2023 - Elsevier
Thermodynamic and kinetic parameters, such as enthalpy, entropy, and free energy, are
crucial in evaluating enzyme stability and activity. These parameters, including the free …
crucial in evaluating enzyme stability and activity. These parameters, including the free …
Preparation, characterization, immobilization, and molecular docking analysis of a novel detergent-stable subtilisin-like serine protease from Streptomyces mutabilis …
We recently described the production of a detergent-biocompatible crude protease from
Streptomyces mutabilis strain TN-X30. Here, we describe the purification, characterization …
Streptomyces mutabilis strain TN-X30. Here, we describe the purification, characterization …
Characterization of a novel protease from Aeribacillus pallidus strain VP3 with potential biotechnological interest
S Mechri, MBE Berrouina, MO Benmrad… - International journal of …, 2017 - Elsevier
The present study investigates the purification and physico-chemical characterization of an
extracellular protease from the Aeribacillus pallidus strain VP3 previously isolated from a …
extracellular protease from the Aeribacillus pallidus strain VP3 previously isolated from a …