In the 1980s, Escherichia coli was the preferred host for heterologous protein expression
owing to its capacity for rapid growth in complex media; well-studied genetics; rapid and …

The kinetics of a thermostable extracellular acid protease produced by an Aspergillus
foetidus strain was investigated at different pH, temperatures and substrate concentrations …

This report describes purification strategies, biochemical properties and thermodynamic
analysis of an alkaline serine protease from a marine actinomycete, Nocardiopsis …

The thermostable crude proteolytic extract and purified protease produced by Aspergillus
tamarii URM4634 were investigated at different temperatures. The activity results were used …

The use of yeasts for the production of proteases has increased in demand in recent years.
Y. lipolytica has been reported as a strain with high yields of protease production. This work …

Bacillus stearothermophilus alkaline protease was conjugated to several oxidized
polysaccharides of different chemical structure. The conjugates were evaluated for the …

The Nocardiopsis alba strain OM-5 showed maximum protease production in submerged
culture. The OM-5 protease was purified by hydrophobic interaction chromatography. The …

Thermodynamic and kinetic parameters, such as enthalpy, entropy, and free energy, are
crucial in evaluating enzyme stability and activity. These parameters, including the free …

We recently described the production of a detergent-biocompatible crude protease from
Streptomyces mutabilis strain TN-X30. Here, we describe the purification, characterization …

The present study investigates the purification and physico-chemical characterization of an
extracellular protease from the Aeribacillus pallidus strain VP3 previously isolated from a …