Sulfatases, which cleave sulfate esters in biological systems, play a key role in regulating
the sulfation states that determine the function of many physiological molecules. Sulfatase …

Formylglycine (fGly) is a catalytically essential residue found almost exclusively in the active
sites of type I sulfatases. Formed by post-translational oxidation of cysteine or serine side …

Enzyme activities (EAs) respond to contamination in several ways depending on the
chemical form and content of heavy metals and metalloids (HMs) and their interactions with …

Directed evolution relies on iterative cycles of randomization and selection. The outcome of
an artificial evolution experiment is crucially dependent on (i) the numbers of variants that …

C α-formylglycine (FGly) is the catalytic residue in the active site of eukaryotic sulfatases. It is
posttranslationally generated from a cysteine in the endoplasmic reticulum. The genetic …

We demonstrate the utility of a microfluidic platform in which water-in-oil droplet
compartments serve to miniaturize cell lysate assays by a million-fold for directed enzyme …

Sulfatases cleave sulfate groups from various molecules and constitute a biologically and
industrially important group of enzymes. However, the number of sulfatases whose substrate …

Kinetic analyses of promiscuous enzymes reveal rate accelerations,(kcat/KM)/k2, of up to
1018 for their secondary activities. Such large values suggest that binding and catalysis can …

Multidrug-resistant (MDR) gram-negative bacteria have increased the prevalence of fatal
sepsis in modern times. Colistin is a cationic antimicrobial peptide (CAMP) antibiotic that …

Human polo‐like kinase Plk1 localizes to the centrosomes, kinetochores and central spindle
structures during mitosis. It plays an essential role in promoting mitosis and cytokinesis …