This manuscript provides a comprehensive review of the peptide and protein identification
process using tandem mass spectrometry (MS/MS) data generated in shotgun proteomic …

Environmental proteomics, the study of changes in the abundance of proteins and their post-
translational modifications, has become a powerful tool for generating hypotheses regarding …

Measuring precise concentrations of proteins can provide insights into biological processes.
Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art …

The subject of this tutorial is protein identification and characterisation by database
searching of MS/MS Data. Peptide Mass Fingerprinting is excluded because it is covered in …

Peptide identification via tandem mass spectrometry sequence database searching is a key
method in the array of tools available to the proteomics researcher. The ability to rapidly and …

The ultimate goal of proteomics is determination of the exact chemical composition of protein
species, including their complete amino acid sequence and the identification of each …

Open modification searching (OMS) is a powerful search strategy that identifies peptides
carrying any type of modification by allowing a modified spectrum to match against its …

The principle of shotgun proteomics is to use peptide mass spectra in order to identify
corresponding sequences in a protein database. The quality of peptide and protein …

This review gives an overview of enzymatic reactions that have been conducted on
substrates attached to solid surfaces. Such biochemical reactions have become more …

Naturally occurring peptides, including growth factors, hormones, and neurotransmitters,
represent an important class of biomolecules and have crucial roles in human physiology …