Phosphoinositides (PIs) make up only a small fraction of cellular phospholipids, yet they
control almost all aspects of a cell's life and death. These lipids gained tremendous research …

Phosphoinositides (PIs) are minor components of cell membranes, but play key roles in cell
function. Recent refinements in techniques for their detection, together with imaging …

Genetically encoded fluorescent sensors are essential tools in modern biological research,
and recent advances in fluorescent proteins (FPs) have expanded the scope of sensor …

Deciphering many roles played by inositol lipids in signal transduction and membrane
function demands experimental approaches that can detect their dynamic accumulation with …

Phosphoinositides constitute a critical family of lipids that regulate numerous cellular
processes. Phosphatidylinositol 4, 5-bisphosphate (PIP2) is arguably the most important …

G Protein-activated K+ channels (GIRK) channels are inhibited by depletion of PtdIns (4, 5)
P 2 (PIP 2), and/or channel phosphorylation by proteinkinase C (PKC). By using FRET …

Anionic phospholipids are essential structural components of cell membranes.
Spatiotemporal dynamics of these lipids play central roles in regulating signalling events …

FRET (Förster Resonance Energy Transfer) microscopy breaks the resolution limit of light to
let us investigate the conformation and function of proteins within living cells. Intensity-based …

Activation of a specific protein kinase C (PKC) isoform during stimulation of G q protein-
coupled receptors (G q PCRs) is determined by homologous receptor desensitization that …

Voltage sensitive phosphatases (VSPs), including engineered voltage sensitive PTEN, are
excellent tools to rapidly and reversibly alter the phosphoinositide (PI) content of the plasma …