HUH endonucleases are numerous and widespread in all three domains of life. The major
function of these enzymes is processing a range of mobile genetic elements by catalysing …

The type IV secretion system (T4SS) VirB/D4 of the facultative intracellular pathogen
Bartonella henselae is known to translocate bacterial effector proteins into human cells. Two …

Bacterial conjugation, a mechanism of horizontal gene transfer, is the major means by which
antibiotic resistance spreads among bacteria (1, 2). Conjugative plasmids are transferred …

Bacterial type IV secretion systems (T4SSs) are involved in processes such as bacterial
conjugation and protein translocation to animal cells. In this work, we have switched the …

Type IV secretion system (T4SS) substrates are recruited through a translocation signal that
is poorly defined for conjugative relaxases. The relaxase TrwC of plasmid R388 is …

We explore the potential of bacterial secretion systems as tools for genomic modification of
human cells. We previously showed that foreign DNA can be introduced into human cells …

Xanthomonas translucens pv. undulosa (Xtu) is a proteobacteria which causes bacterial leaf
streak (BLS) or bacterial chaff disease in wheat and barley. The constant competition for zinc …

Relaxases act as DNA selection sieves in conjugative plasmid transfer. Most plasmid
relaxases belong to the HUH endonuclease family. TrwC, the relaxase of plasmid R388, is …

Site-specific recombinases (SSRs) have been crucial in the development of mammalian
transgenesis. For gene therapy purposes, this approach remains challenging, because, for …

Bacterial conjugation is a mechanism of horizontal DNA transfer. The relaxase TrwC of the
conjugative plasmid R388 cleaves one strand of the transferred DNA at the oriT gene …