Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
SA Bustin - Journal of molecular endocrinology, 2000 - jme.bioscientifica.com
To investigate whether the tumor suppressor gene PTEN affects the activity of the androgen
receptor (AR), we monitored the expression of the apoptotic gene HA-Bax (inserted in an …
receptor (AR), we monitored the expression of the apoptotic gene HA-Bax (inserted in an …
Activation of HIV transcription with short-course vorinostat in HIV-infected patients on suppressive antiretroviral therapy
JH Elliott, F Wightman, A Solomon, K Ghneim… - PLoS …, 2014 - journals.plos.org
Human immunodeficiency virus (HIV) persistence in latently infected resting memory CD4+
T-cells is the major barrier to HIV cure. Cellular histone deacetylases (HDACs) are important …
T-cells is the major barrier to HIV cure. Cellular histone deacetylases (HDACs) are important …
A novel assay to measure the magnitude of the inducible viral reservoir in HIV-infected individuals
FA Procopio, R Fromentin, DA Kulpa, JH Brehm… - …, 2015 - thelancet.com
Background Quantifying latently infected cells is critical to evaluate the efficacy of
therapeutic strategies aimed at reducing the size of the long-lived viral reservoir, but the low …
therapeutic strategies aimed at reducing the size of the long-lived viral reservoir, but the low …
Real-time PCR in virology
IM Mackay, KE Arden, A Nitsche - Nucleic acids research, 2002 - academic.oup.com
The use of the polymerase chain reaction (PCR) in molecular diagnostics has increased to
the point where it is now accepted as the gold standard for detecting nucleic acids from a …
the point where it is now accepted as the gold standard for detecting nucleic acids from a …
An overview of real-time quantitative PCR: applications to quantify cytokine gene expression
A Giulietti, L Overbergh, D Valckx, B Decallonne… - Methods, 2001 - Elsevier
The analysis of cytokine profiles helps to clarify functional properties of immune cells, both
for research and for clinical diagnosis. The real-time reverse transcription polymerase chain …
for research and for clinical diagnosis. The real-time reverse transcription polymerase chain …
[HTML][HTML] Real-time PCR in the microbiology laboratory
IM Mackay - Clinical microbiology and infection, 2004 - Elsevier
Use of PCR in the field of molecular diagnostics has increased to the point where it is now
accepted as the standard method for detecting nucleic acids from a number of sample and …
accepted as the standard method for detecting nucleic acids from a number of sample and …
Bryostatin-1 for latent virus reactivation in HIV-infected patients on antiretroviral therapy
C Gutiérrez, S Serrano-Villar, N Madrid-Elena… - Aids, 2016 - journals.lww.com
Objective: The protein kinase C (PKC) agonist bryostatin-1 has shown significant ex-vivo
potency to revert HIV-1 latency, compared with other latency reversing agents (LRA). The …
potency to revert HIV-1 latency, compared with other latency reversing agents (LRA). The …
The HIV-1 reservoir in eight patients on long-term suppressive antiretroviral therapy is stable with few genetic changes over time
L Josefsson, S Von Stockenstrom… - Proceedings of the …, 2013 - National Acad Sciences
The source and dynamics of persistent HIV-1 during long-term combinational antiretroviral
therapy (cART) are critical to understanding the barriers to curing HIV-1 infection. To …
therapy (cART) are critical to understanding the barriers to curing HIV-1 infection. To …
Low-level viremia persists for at least 7 years in patients on suppressive antiretroviral therapy
S Palmer, F Maldarelli, A Wiegand… - Proceedings of the …, 2008 - National Acad Sciences
Residual viremia can be detected in most HIV-1-infected patients on antiretroviral therapy
despite suppression of plasma RNA to< 50 copies per ml, but the source and duration of this …
despite suppression of plasma RNA to< 50 copies per ml, but the source and duration of this …