Principles of genome folding into topologically associating domains

Q Szabo, F Bantignies, G Cavalli - Science advances, 2019 - science.org
Understanding the mechanisms that underlie chromosome folding within cell nuclei is
essential to determine the relationship between genome structure and function. The recent …

Control of DNA replication timing in the 3D genome

C Marchal, J Sima, DM Gilbert - Nature Reviews Molecular Cell Biology, 2019 - nature.com
The 3D organization of mammalian chromatin was described more than 30 years ago by
visualizing sites of DNA synthesis at different times during the S phase of the cell cycle …

Predicting 3D genome folding from DNA sequence with Akita

G Fudenberg, DR Kelley, KS Pollard - Nature methods, 2020 - nature.com
In interphase, the human genome sequence folds in three dimensions into a rich variety of
locus-specific contact patterns. Cohesin and CTCF (CCCTC-binding factor) are key …

Highly rearranged chromosomes reveal uncoupling between genome topology and gene expression

Y Ghavi-Helm, A Jankowski, S Meiers, RR Viales… - Nature …, 2019 - nature.com
Chromatin topology is intricately linked to gene expression, yet its functional requirement
remains unclear. Here, we comprehensively assessed the interplay between genome …

Topological structures and syntenic conservation in sea anemone genomes

B Zimmermann, JD Montenegro, SMC Robb… - Nature …, 2023 - nature.com
There is currently little information about the evolution of gene clusters, genome
architectures and karyotypes in early branching animals. Slowly evolving anthozoan …

CTCF shapes chromatin structure and gene expression in health and disease

B Dehingia, M Milewska, M Janowski, A Pękowska - EMBO reports, 2022 - embopress.org
CCCTC‐binding factor (CTCF) is an eleven zinc finger (ZF), multivalent transcriptional
regulator, that recognizes numerous motifs thanks to the deployment of distinct combinations …

Joint profiling of DNA methylation and chromatin architecture in single cells

G Li, Y Liu, Y Zhang, N Kubo, M Yu, R Fang, M Kellis… - Nature …, 2019 - nature.com
We report a molecular assay, Methyl-HiC, that can simultaneously capture the chromosome
conformation and DNA methylome in a cell. Methyl-HiC reveals coordinated DNA …

Functional dissection of the Sox9Kcnj2 locus identifies nonessential and instructive roles of TAD architecture

A Despang, R Schöpflin, M Franke, S Ali, I Jerković… - Nature …, 2019 - nature.com
The genome is organized in three-dimensional units called topologically associating
domains (TADs), through a process dependent on the cooperative action of cohesin and the …

Multi-feature clustering of CTCF binding creates robustness for loop extrusion blocking and Topologically Associating Domain boundaries

LH Chang, S Ghosh, A Papale, JM Luppino… - Nature …, 2023 - nature.com
Abstract Topologically Associating Domains (TADs) separate vertebrate genomes into
insulated regulatory neighborhoods that focus genome-associated processes. TADs are …

Emerging evidence of chromosome folding by loop extrusion

G Fudenberg, N Abdennur, M Imakaev… - Cold Spring Harbor …, 2017 - symposium.cshlp.org
Chromosome organization poses a remarkable physical problem with many biological
consequences: How can molecular interactions between proteins at the nanometer scale …