In the early 2000s, Tawfik presented his 'New View'on enzyme evolution, highlighting the
role of conformational plasticity in expanding the functional diversity of limited repertoires of …

The β-lactams are the most widely used antibacterial agents worldwide. These antibiotics, a
group that includes the penicillins and cephalosporins, are covalent inhibitors that target …

Epistasis, the non-additive effect of mutations, can provide combinatorial improvements to
enzyme activity that substantially exceed the gains from individual mutations. Yet the …

Antimicrobial resistance (AMR) in bacteria is a major threat to public health; one of the key
elements in the spread and evolution of AMR in clinical pathogens is the transfer of …

Background Cefiderocol is a novel siderophore β-lactam with improved hydrolytic stability
toward β-lactamases, including carbapenemases, achieved by combining structural …

Biocatalysts that are eco-friendly, sustainable, and highly specific have great potential for
applications in the production of fine chemicals, food, detergents, biofuels, pharmaceuticals …

Antibacterial resistance towards the β-lactam (BL) drugs is now ubiquitous, and there is a
major global health concern associated with the emergence of new β-lactamases (BLAs) as …

By providing the bacterial cell with protection against several antibiotics at once,
multiresistance plasmids have an evolutionary advantage in situations where antibiotic …

Major antibiotic groups are losing effectiveness due to the uncontrollable spread of
antimicrobial resistance (AMR) genes. Among these, β-lactam resistance genes–encoding β …

Abstract β-Lactamases represent one of the most prevalent resistance mechanisms against
β-lactam antibiotics. Beyond their clinical importance, they have also become key models in …