The correlation between mRNA and protein abundances in the cell has been reported to be
notoriously poor. Recent technological advances in the quantitative analysis of mRNA and …

Cellular states are determined by differential expression of the cell's proteins. The
relationship between protein and mRNA expression levels informs about the combined …

Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …

Data on absolute molecule numbers will empower the modeling, understanding, and
comparison of cellular functions and biological systems. We quantified transcriptomes and …

Synonymous mutations do not alter the protein produced yet can have a significant effect on
protein levels. The mechanisms by which this effect is achieved are controversial; although …

Background Microarrays revolutionized biological research by enabling gene expression
comparisons on a transcriptome-wide scale. Microarrays, however, do not estimate absolute …

Cells respond to their environment by modulating protein levels through mRNA transcription
and post-transcriptional control. Modest observed correlations between global steady-state …

To quantify cell cycle-dependent fluctuations on a proteome-wide scale, we performed
integrative analysis of the proteome and phosphoproteome during the four major phases of …

We describe the first large scale analysis of gene translation that is based on a model that
takes into account the physical and dynamical nature of this process. The Ribosomal Flow …

Studies of the Plasmodium falciparum transcriptome have shown that the tightly controlled
progression of the parasite through the intra-erythrocytic developmental cycle (IDC) is …