Pullulanases are well‐known debranching enzymes hydrolyzing α‐1, 6‐glycosidic linkages.
To date, engineering of pullulanase is mainly focused on catalytic pocket or domain tailoring …

Abstract β‐glucosidases play a pivotal role in second‐generation biofuel (2G‐biofuel)
production. For this application, thermostable enzymes are essential due to the denaturing …

Β-glucosidases are key enzymes used in second-generation biofuel production. They act in
the last step of the lignocellulose saccharification, converting cellobiose in glucose …

Evolutionarily related proteins can present similar structures but very dissimilar sequences.
Hence, understanding the role of the inter-residues contacts for the protein structure has …

The active site residues in GH1 β-glycosidases are compartmentalized into 3 functional
regions, involved in catalysis or binding of glycone and aglycone motifs from substrate …

Ent-copalyl diphosphate synthase controls the biosynthesis of gibberellin plant hormones,
which in turn coordinate the expression of numerous enzymes. Some gibberellin-dependent …

Salt bridges are the strongest electrostatic interactions in proteins. They substantially
contribute to a protein's structural stability. Thus, mutations of salt bridges are typically …

Protein engineering is actively pursued in industrial and laboratory settings for high
thermostability. Among the many protein engineering methods, rational design by …

The enzymatic hydrolysis of cellulose and lignocellulosic materials is marked by a rate
decrease along the reaction time. Cellobiohydrolase slow dissociation from the substrate …

Evolutionarily related proteins can present similar structures but very dissimilar sequences.
Hence, understanding the role of the inter-residues contacts for the protein structure has …