Cellular pathways that repair chromosomal double-strand breaks (DSBs) have pivotal roles
in cell growth, development and cancer. These DSB repair pathways have been the target of …

Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

Conventional CRISPR–Cas systems maintain genomic integrity by leveraging guide RNAs
for the nuclease-dependent degradation of mobile genetic elements, including plasmids and …

Abstract Homology-directed repair (HDR), a method for repair of DNA double-stranded
breaks can be leveraged for the precise introduction of mutations supplied by synthetic DNA …

CRISPR-Cas9 is a promising technology for genome editing. Here we use Cas9 nuclease-
induced double-strand break DNA (DSB) at the UROS locus to model and correct congenital …

DNA double-strand breaks arise accidentally upon exposure of DNA to radiation and
chemicals or result from faulty DNA metabolic processes. DNA breaks can also be …

Prime editors (PEs) enable targeted precise editing, including the generation of
substitutions, insertions and deletions, in eukaryotic genomes. However, their genome-wide …

Genome-embedded ribonucleotides arrest replicative DNA polymerases (Pols) and cause
DNA breaks. Whether mammalian DNA repair Pols efficiently use template ribonucleotides …

The number of DNA polymerases identified in each organism has mushroomed in the past
two decades. Most newly found DNA polymerases specialize in translesion synthesis and …

Polymerase theta (Pol θ, gene name Polq) is a widely conserved DNA polymerase that
mediates a microhomology-mediated, error-prone, double strand break (DSB) repair …