Quantification and mapping of DNA modifications

Y Dai, BF Yuan, YQ Feng - RSC chemical biology, 2021 - pubs.rsc.org
Apart from the four canonical nucleobases, DNA molecules carry a number of natural
modifications. Substantial evidence shows that DNA modifications can regulate diverse …

[HTML][HTML] Methods for Detection and Mapping of Methylated and Hydroxymethylated Cytosine in DNA

O Kisil, A Sergeev, A Bacheva, M Zvereva - Biomolecules, 2024 - mdpi.com
The chemical modifications of DNA are of pivotal importance in the epigenetic regulation of
cellular processes. Although the function of 5-methylcytosine (5mC) has been extensively …

Single-molecule quantification of 5-hydroxymethylcytosine for diagnosis of blood and colon cancers

N Gilat, T Tabachnik, A Shwartz, T Shahal… - Clinical …, 2017 - Springer
Background The DNA modification 5-hydroxymethylcytosine (5hmC) is now referred to as
the sixth base of DNA with evidence of tissue-specific patterns and correlation with gene …

Quantification of global DNA hydroxymethylation level using UHRF2 SRA-luciferase based on bioluminescence resonance energy transfer

N Taka, S Asami, M Sakamoto, T Matsui… - Analytical …, 2022 - ACS Publications
5-Methylcytosine (5mC) plays an important role in the regulation of gene expression. Ten-
eleven translocation (TET) continuously oxidizes 5mC to 5-hydroxymethylcytosine (5hmC), 5 …

Hypersensitive quantification of global 5-hydroxymethylcytosine by chemoenzymatic tagging

T Shahal, O Koren, G Shefer, N Stern… - Analytica Chimica Acta, 2018 - Elsevier
hydroxymethylcytosine (5hmC) is an epigenetic DNA modification. Tissue-specific reduction
in global 5hmC levels has been associated with various types of cancer. One of the …

Dual-monomer solvatochromic probe system (DSPS) for effectively differentiating lipid raft cholesterol and active membrane cholesterol in the inner-leaflet plasma …

X Luo, Y Zhao, Y Cai, J Chen, L Zhao, T Lan… - Journal of Materials …, 2024 - pubs.rsc.org
Monitoring active membrane cholesterol and lipid raft cholesterol in the inner leaflet of the
plasma membrane is significant for understanding the membrane function and cellular …

[HTML][HTML] Cell-Free DNA Hydroxymethylation in Cancer: Current and Emerging Detection Methods and Clinical Applications

JJN Li, G Liu, BH Lok - Genes, 2024 - mdpi.com
In the era of precision oncology, identifying abnormal genetic and epigenetic alterations has
transformed the way cancer is diagnosed, managed, and treated. 5-hydroxymethylcytosine …

Quantitation and mapping of the epigenetic marker 5‐hydroxymethylcytosine

Y Qing, Z Tian, Y Bi, Y Wang, J Long, CX Song… - …, 2017 - Wiley Online Library
We here review primary methods used in quantifying and mapping 5‐hydroxymethylcytosine
(5hmC), including global quantification, restriction enzyme‐based detection, and methods …

Irys extract

R Arielly, Y Ebenstein - Bioinformatics, 2018 - academic.oup.com
Irys Extract is a software tool for generating genomic information from data collected by the
BioNano Genomics Irys platform. The tool allows the user easy access to the raw data in the …

[PDF][PDF] Chemical biology

J Bai, F Guo, M Li, Y Li, X Lei - 2021 - pdfs.semanticscholar.org
We here describe a fluorescent signal amplification method termed ''Click-based
amplification''that can be well integrated with various click-labelling modes, including …