Attaining true quantitative data from WB requires that all the players involved in the
procedure are quality controlled including the user. Appropriate protein extraction method …

Western blotting is an analytical method widely used for detecting and (semi‐) quantifying
specific proteins in given samples. Western blots are continuously applied and developed …

The artificial lung (AL) technology is one of the membrane-based artificial organs that partly
augments lung functions, ie blood oxygenation and CO 2 removal. It is generally employed …

Western blotting (WB), also known as immunoblotting, is a well-known molecular biology
method that biologists often use to investigate many features of the protein, ranging from …

Fluorescent transcriptional reporters are widely used as signaling reporters and biomarkers
to monitor pathway activities and determine cell type identities. However, a large amount of …

Hepatocellular carcinoma (HCC) is a liver tumor that usually arises in patients with cirrhosis.
Hepatic stellate cells are key players in the progression of HCC, as they create a fibrotic …

Abstract Spinal Muscular Atrophy (SMA) is caused by mutation or deletion of the survival
motor neuron 1 (SMN1) gene. Decreased levels of, cell-ubiquitous, SMN protein is …

Wee1 kinase is a crucial negative regulator of Cdk1/cyclin B1 activity and is required for
normal entry into and exit from mitosis. Wee1 activity can be chemically inhibited by the …

Coagulation factor V (FV) plays an anticoagulant role but serves as a procoagulant cofactor
in the prothrombinase complex once activated to FVa. At the heart of these opposing effects …

Western blotting experiments were initially performed to detect a target protein in a complex
biological sample and more recently, to measure relative protein abundance …