Applying a high voltage to a liquid passing through a capillary disintegrates the liquid
meniscus to generate a fine spray of charged droplets. As the droplets evaporate, polar …

Mass spectrometry is a central technology in the life sciences, providing our most
comprehensive account of the molecular inventory of the cell. In parallel with developments …

Adeno-associated viral (AAV) vectors have emerged as gene therapy and vaccine delivery
systems. Differential scanning fluorimetry or differential scanning calorimetry is commonly …

Mass spectrometry (MS), a century-old analytical technique, is now emerging as a
preparative method. Electrospray deposition (ESD) of charged droplets generated by …

Chaperonins are nanomachines that harness ATP hydrolysis to power and catalyze protein
folding, a chemical action that is directly linked to the maintenance of cell function through …

Lipids are critical modulators of membrane protein structure and function. However, it is
challenging to investigate the thermodynamics of protein–lipid interactions because lipids …

Proteins can adopt different conformational states that are important for their biological
function and, in some cases, can be responsible for their dysfunction. The essential roles …

As the COVID-19 pathogen, SARS-CoV-2 relies on its main protease (MPro) for
pathogenesis and replication. During crystallographic analyses of MPro crystals that were …

The recent discovery of asymmetric arrangements of trimers in the tautomerase superfamily
(TSF) adds structural diversity to this already mechanistically diverse superfamily …

Variable-temperature electrospray ionization (vT-ESI) native mass spectrometry (nMS) is
used to determine the thermodynamics for stepwise binding of up to 14 ATP molecules to …