From 2D to 3D cancer cell models—the enigmas of drug delivery research

I Van Zundert, B Fortuni, S Rocha - Nanomaterials, 2020 - mdpi.com
Over the past decades, research has made impressive breakthroughs towards drug delivery
systems, resulting in a wide range of multifunctional engineered nanoparticles with …

Proteinaceous hydrogels for bioengineering advanced 3D tumor models

B Blanco‐Fernandez, VM Gaspar, E Engel… - Advanced …, 2021 - Wiley Online Library
The establishment of tumor microenvironment using biomimetic in vitro models that
recapitulate key tumor hallmarks including the tumor supporting extracellular matrix (ECM) is …

[HTML][HTML] Animal-derived products in science and current alternatives

AC Duarte, EC Costa, HAL Filipe, SM Saraiva… - Biomaterials …, 2023 - Elsevier
More than fifty years after the 3Rs definition and despite the continuous implementation of
regulatory measures, animals continue to be widely used in basic research. Their use …

Integrated cancer tissue engineering models for precision medicine

ME Bregenzer, EN Horst, P Mehta, CM Novak… - PLoS …, 2019 - journals.plos.org
Tumors are not merely cancerous cells that undergo mindless proliferation. Rather, they are
highly organized and interconnected organ systems. Tumor cells reside in complex …

Host metabolites stimulate the bacterial proton motive force to enhance the activity of aminoglycoside antibiotics

A Crabbe, L Ostyn, S Staelens, C Rigauts… - PLoS …, 2019 - journals.plos.org
Antibiotic susceptibility of bacterial pathogens is typically evaluated using in vitro assays that
do not consider the complex host microenvironment. This may help explaining a significant …

Influences of the 3D microenvironment on cancer cell behaviour and treatment responsiveness: A recent update on lung, breast and prostate cancer models

LS Costard, RR Hosn, H Ramanayake, FJ O'Brien… - Acta Biomaterialia, 2021 - Elsevier
The majority of in vitro studies assessing cancer treatments are performed in two-
dimensional (2D) monolayers and are subsequently validated in in vivo animal models …

[HTML][HTML] Graphene inks for the 3D printing of cell culture scaffolds and related molecular arrays

GM Vlăsceanu, H Iovu, M Ioniţă - Composites Part B: Engineering, 2019 - Elsevier
Abstract 3D printing technology has changed academia and industry perception regarding
the course of evolution in the manufacturing of customizable products, paving the way …

Recent progress in 3d printing of 2d material‐based macrostructures

R Yang, J Zhou, C Yang, L Qiu… - Advanced Materials …, 2020 - Wiley Online Library
Abstract 2D materials have a range of unique properties and are promising building blocks
for the fabrication of macroscopic materials for many applications ranging from flexible …

Advances in 3D peptide hydrogel models in cancer research

J Xu, G Qi, W Wang, XS Sun - npj Science of Food, 2021 - nature.com
In vitro cell culture models on monolayer surfaces (2D) have been widely adapted for
identification of chemopreventive food compounds and food safety evaluation. However, the …

Collagen content and extracellular matrix cause cytoskeletal remodelling in pancreatic fibroblasts

A Stylianou, V Gkretsi, M Louca… - Journal of the …, 2019 - royalsocietypublishing.org
In many solid tumours a desmoplastic reaction takes place, which results in tumour tissue
stiffening due to the extensive production of extracellular matrix (ECM) proteins, such as …