Bringing new methods to the seed proteomics platform: challenges and perspectives

G Smolikova, D Gorbach, E Lukasheva… - International journal of …, 2020 - mdpi.com
For centuries, crop plants have represented the basis of the daily human diet. Among them,
cereals and legumes, accumulating oils, proteins, and carbohydrates in their seeds …

Concepts and strategies of soybean seed proteomics using the shotgun proteomics approach

CW Min, R Gupta, GK Agrawal, R Rakwal… - Expert Review of …, 2019 - Taylor & Francis
Introduction: The last decade has yielded significant developments in the field of proteomics,
especially in mass spectrometry (MS) and data analysis tools. In particular, a shift from gel …

In-depth proteomic analysis of Glycine max seeds during controlled deterioration treatment reveals a shift in seed metabolism

CW Min, SH Lee, YE Cheon, WY Han, JM Ko… - Journal of …, 2017 - Elsevier
Seed aging is one of the major events, affecting the overall quality of agricultural seeds. To
analyze the effect of seed aging, soybean seeds were exposed to controlled deterioration …

Protein extraction methods shape much of the extracted proteomes

L Niu, H Yuan, F Gong, X Wu, W Wang - Frontiers in Plant Science, 2018 - frontiersin.org
Recently, technical advances, especially in liquid chromatography (LC) and mass
spectrometry (MS), have improved the sensitivity, coverage, reliability, and throughput of …

[HTML][HTML] A practical introduction to holo-omics

I Odriozola, JA Rasmussen, MTP Gilbert… - Cell Reports …, 2024 - cell.com
Holo-omics refers to the joint study of non-targeted molecular data layers from host-
microbiota systems or holobionts, which is increasingly employed to disentangle the …

In-Depth Investigation of Low-Abundance Proteins in Matured and Filling Stages Seeds of Glycine max Employing a Combination of Protamine Sulfate Precipitation …

CW Min, J Park, JW Bae, GK Agrawal, R Rakwal, Y Kim… - Cells, 2020 - mdpi.com
Despite the significant technical advancements in mass spectrometry-based proteomics and
bioinformatics resources, dynamic resolution of soybean seed proteome is still limited …

Integrated proteomics and metabolomics analysis highlights correlative metabolite-protein networks in soybean seeds subjected to warm-water soaking

CW Min, H Hyeon, R Gupta, J Park… - Journal of Agricultural …, 2020 - ACS Publications
Soaking of soybean seeds is a prerequisite for the production of soy foods, and it has been
shown that the extent of water absorbed during different imbibition conditions directly affects …

Coupling of gel-based 2-DE and 1-DE shotgun proteomics approaches to dig deep into the leaf senescence proteome of Glycine max

R Gupta, SJ Lee, CW Min, SW Kim, KH Park… - Journal of …, 2016 - Elsevier
Leaf senescence is the last stage of leaf development that re-mobilizes nutrients from the
source to sink. Here, we have utilized the soybean as a model system to unravel …

Proteomic Analysis of Ginseng (Panax ginseng CA Meyer) Fluid Proteins under Salt Stress

JY Jung, CW Min, SW Kim, R Gupta, W Jang, KH Bang… - Agronomy, 2022 - mdpi.com
Ginseng (Panax ginseng CA Meyer), due to its relatively longer cultivation time, is often
exposed to environmental stresses such as heat, salt, and drought. Particularly, salt-stress …

Comparative biochemical and proteomic analyses of soybean seed cultivars differing in protein and oil content

CW Min, R Gupta, SW Kim, SE Lee… - Journal of agricultural …, 2015 - ACS Publications
This study develops differential protein profiles of soybean (Glycine max) seeds (cv.
Saedanbaek and Daewon) varying in protein (47.9 and 39.2%) and oil (16.3 and 19.7%) …