Inteins in science: evolution to application
Inteins are mobile genetic elements that apply standard enzymatic strategies to excise
themselves post-translationally from the precursor protein via protein splicing. Since their …
themselves post-translationally from the precursor protein via protein splicing. Since their …
Metal effect on intein splicing: A review
Inteins are intervening polypeptides that interrupt the functional domains of several
important proteins across the three domains of life. Inteins excise themselves from the …
important proteins across the three domains of life. Inteins excise themselves from the …
The crystal structure of the naturally split gp41‐1 intein guides the engineering of orthogonal split inteins from cis‐splicing inteins
Protein trans‐splicing catalyzed by split inteins has increasingly become useful as a protein
engineering tool. We solved the 1.0 Å‐resolution crystal structure of a fused variant from the …
engineering tool. We solved the 1.0 Å‐resolution crystal structure of a fused variant from the …
Structural and biochemical analysis of a novel atypically split intein reveals a conserved histidine specific to cysteine-less inteins
T Pasch, A Schröder, S Kattelmann, M Eisenstein… - Chemical …, 2023 - pubs.rsc.org
Protein trans-splicing mediated by a split intein reconstitutes a protein backbone from two
parts. This virtually traceless autoprocessive reaction provides the basis for numerous …
parts. This virtually traceless autoprocessive reaction provides the basis for numerous …
Conditional protein splicing switch in hyperthermophiles through an intein-extein partnership
Inteins are intervening proteins that undergo an autocatalytic splicing reaction that ligates
flanking host protein sequences termed exteins. Some intein-containing proteins have …
flanking host protein sequences termed exteins. Some intein-containing proteins have …
Substrate specificities of inteins investigated by QuickDrop‐cassette mutagenesis
Inteins catalyze self‐excision from host precursor proteins while concomitantly ligating the
flanking substrates (exteins) with a peptide bond. Noncatalytic extein residues near the …
flanking substrates (exteins) with a peptide bond. Noncatalytic extein residues near the …
Crystal structures of CDC21-1 inteins from hyperthermophilic archaea reveal the selection mechanism for the highly conserved homing endonuclease insertion site
Self-splicing inteins are mobile genetic elements invading host genes via nested homing
endonuclease (HEN) domains. All HEN domains residing within inteins are inserted at a …
endonuclease (HEN) domains. All HEN domains residing within inteins are inserted at a …
Structural basis for the propagation of homing endonuclease-associated inteins
HM Beyer, H Iwaï - Frontiers in Molecular Biosciences, 2022 - frontiersin.org
Inteins catalyze their removal from a host protein through protein splicing. Inteins that
contain an additional site-specific endonuclease domain display genetic mobility via a …
contain an additional site-specific endonuclease domain display genetic mobility via a …
Neighboring inteins interfere with one another's homing capacity
I Turgeman-Grott, D Arsenault, D Yahav, Y Feng… - PNAS …, 2023 - academic.oup.com
Inteins are mobile genetic elements that invade conserved genes across all domains of life
and viruses. In some instances, a single gene will have several intein insertion sites. In …
and viruses. In some instances, a single gene will have several intein insertion sites. In …
Novel protein ligase based on dual split intein
B Lei, S Wang, X Zhang, T Chen, Y Lin - Biochemical and Biophysical …, 2024 - Elsevier
Inteins are unique single-turnover enzymes that can excise themselves from the precursor
protein without the aid of any external cofactors or energy. In most cases, inteins are …
protein without the aid of any external cofactors or energy. In most cases, inteins are …