Cryo-electron microscopy in the study of virus entry and infection

M Dutta, P Acharya - Frontiers in Molecular Biosciences, 2024 - frontiersin.org
Viruses have been responsible for many epidemics and pandemics that have impacted
human life globally. The COVID-19 pandemic highlighted both our vulnerability to viral …

Antibody Data Analysis from Diverse Immune Libraries

AS Fahad, MF Gutiérrez-Gonzalez… - Cold Spring Harbor …, 2024 - cshprotocols.cshlp.org
Antibody functional screening studies and next-generation sequencing require careful
processing and interpretation of sequence data for optimal results. Here, we provide a …

Vaccine-elicited and naturally elicited antibodies differ in their recognition of the HIV-1 fusion peptide

M Reveiz, K Xu, M Lee, S Wang, AS Olia… - Frontiers in …, 2024 - frontiersin.org
Broadly neutralizing antibodies have been proposed as templates for HIV-1 vaccine design,
but it has been unclear how similar vaccine-elicited antibodies are to their naturally elicited …

Beyond Single Clones: High-Throughput Sequencing in Antibody Discovery

AS Fahad, MF Gutiérrez-Gonzalez… - Cold Spring Harbor …, 2024 - cshprotocols.cshlp.org
Antibody repertoire sequencing and display library screening are powerful approaches for
antibody discovery and engineering that can connect DNA sequence with antibody function …

A library-on-library screen reveals the breadth expansion landscape of a broadly neutralizing betacoronavirus antibody

MY Ornelas, WO Ouyang, NC Wu - bioRxiv, 2024 - biorxiv.org
Broadly neutralizing antibodies (bnAbs) typically evolve cross-reactivity breadth through
acquiring somatic hypermutations. While evolution of breadth requires improvement of …