High-throughput analysis of hazards in novel food based on the density functional theory and multimodal deep learning

L Shi, W Jia, R Zhang, Z Fan, W Bian, H Mo - Food Chemistry, 2024 - Elsevier
The emergence of cultured meat presents the potential for personalized food additive
manufacturing, offering a solution to address future food resource scarcity. Processing raw …

Digital Microfluidics for Sample Preparation in Low‐Input Proteomics

MK Steinbach, J Leipert, T Matzanke… - Small Methods, 2024 - Wiley Online Library
Low‐input proteomics, also referred to as micro‐or nanoproteomics, has become
increasingly popular as it allows one to elucidate molecular processes in rare biological …

Automated imaging and identification of proteoforms directly from ovarian cancer tissue

JP McGee, P Su, KR Durbin, MAR Hollas… - Nature …, 2023 - nature.com
The molecular identification of tissue proteoforms by top-down mass spectrometry (TDMS) is
significantly limited by throughput and dynamic range. We introduce AutoPiMS, a single-ion …

Influence of different sample preparation approaches on proteoform identification by top-down proteomics

PT Kaulich, K Jeong, O Kohlbacher, A Tholey - Nature Methods, 2024 - nature.com
Top-down proteomics using mass spectrometry facilitates the identification of intact
proteoforms, that is, all molecular forms of proteins. Multiple past advances have lead to the …

Digital Microfluidics and Magnetic Bead‐Based Intact Proteoform Elution for Quantitative Top‐down Nanoproteomics of Single C. elegans Nematodes

J Leipert, PT Kaulich, MK Steinbach… - Angewandte Chemie …, 2023 - Wiley Online Library
While most nanoproteomics approaches for the analysis of low‐input samples are based on
bottom‐up proteomics workflows, top‐down approaches enabling proteoform …

Recent advancements in subcellular proteomics: growing impact of organellar protein niches on the understanding of cell biology

V Bhushan, A Nita-Lazar - Journal of Proteome Research, 2024 - ACS Publications
The mammalian cell is a complex entity, with membrane-bound and membrane-less
organelles playing vital roles in regulating cellular homeostasis. Organellar protein niches …

Top-down proteomics

DS Roberts, JA Loo, YO Tsybin, X Liu, S Wu… - Nature Reviews …, 2024 - nature.com
Proteoforms, which arise from post-translational modifications, genetic polymorphisms and
RNA splice variants, play a pivotal role as drivers in biology. Understanding proteoforms is …

Top-Down Proteomics Analysis of Picogram-Level Complex Samples Using Spray-Capillary-Based Capillary Electrophoresis–Mass Spectrometry

Z Zhao, Y Guo, T Chowdhury, S Anjum, J Li… - Analytical …, 2024 - ACS Publications
Proteomics analysis of mass-limited samples has become increasingly important for
understanding biological systems in physiologically relevant contexts such as patient …

Discovery top-down proteomics in symbiotic soybean root nodules

M Zhou, JM Fulcher, KJ Zemaitis, DJ Degnan… - Frontiers in Analytical …, 2022 - frontiersin.org
Proteomic methods have been widely used to study proteins in complex biological samples
to understand biological molecular mechanisms. Most well-established methods (known as …

Laser capture microdissection and native mass spectrometry for spatially-resolved analysis of intact protein assemblies in tissue

JW Hughes, EK Sisley, OJ Hale, HJ Cooper - Chemical Science, 2024 - pubs.rsc.org
Previously, we have shown that native ambient mass spectrometry imaging allows the
spatial mapping of folded proteins and their complexes in thin tissue sections. Subsequent …