Microtubules are core components of the eukaryotic cytoskeleton with essential roles in cell
division, shaping, motility and intracellular transport. Despite their functional heterogeneity …

Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction
limit—in light microscopy and enabled visualization of previously invisible molecular details …

Determining the structure and composition of macromolecular assemblies is a major
challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an …

The axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells. In
humans, impaired axoneme function causes a range of ciliopathies. Axoneme assembly …

Cryofixation has proven to be the gold standard for efficient preservation of native cell
ultrastructure compared to chemical fixation, but this approach is not widely used in …

Expansion microscopy (ExM) enables super-resolution fluorescence imaging of physically
expanded biological samples with conventional microscopes. By combining ExM with single …

Centrioles are microtubule-based organelles responsible for forming centrosomes and cilia,
which serve as microtubule-organizing, signaling, and motility centers. Biogenesis and …

Stimulated emission depletion (STED) microscopy is routinely used to resolve the
ultrastructure of cells with a∼ 10-fold higher resolution compared to diffraction limited …

Expansion microscopy (ExM) physically magnifies specimens, allowing to obtain super-
resolution images using a conventional diffraction-limited microscope such as confocal …

The centriole organelle consists of microtubules (MTs) that exhibit a striking 9-fold radial
symmetry. Centrioles play fundamental roles across eukaryotes, notably in cell signaling …