Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Three-dimensional localization of single molecules for super-resolution imaging and single-particle tracking

L von Diezmann, Y Shechtman, WE Moerner - Chemical reviews, 2017 - ACS Publications
Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …

Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes

F Balzarotti, Y Eilers, KC Gwosch, AH Gynnå… - Science, 2017 - science.org
We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the
emitter with a local intensity minimum of excitation light, MINFLUX minimizes the …

A review of progress in single particle tracking: from methods to biophysical insights

C Manzo, MF Garcia-Parajo - Reports on progress in physics, 2015 - iopscience.iop.org
Optical microscopy has for centuries been a key tool to study living cells with minimum
invasiveness. The advent of single molecule techniques over the past two decades has …

Switchable fluorophores for single-molecule localization microscopy

H Li, JC Vaughan - Chemical reviews, 2018 - ACS Publications
The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Photoswitching fingerprint analysis bypasses the 10-nm resolution barrier

DA Helmerich, G Beliu, D Taban, M Meub, M Streit… - Nature …, 2022 - nature.com
Advances in super-resolution microscopy have demonstrated single-molecule localization
precisions of a few nanometers. However, translation of such high localization precisions …

The 2015 super-resolution microscopy roadmap

SW Hell, SJ Sahl, M Bates, X Zhuang… - Journal of Physics D …, 2015 - iopscience.iop.org
Far-field optical microscopy using focused light is an important tool in a number of scientific
disciplines including chemical,(bio) physical and biomedical research, particularly with …

Stimulated emission depletion microscopy

H Blom, J Widengren - Chemical reviews, 2017 - ACS Publications
Despite its short history, diffraction-unlimited fluorescence microscopy techniques have
already made a substantial imprint in the biological sciences. In this review, we describe …

Precisely and accurately localizing single emitters in fluorescence microscopy

H Deschout, FC Zanacchi, M Mlodzianoski, A Diaspro… - Nature …, 2014 - nature.com
Methods based on single-molecule localization and photophysics have brought nanoscale
imaging with visible light into reach. This has enabled single-particle tracking applications …

Super-resolution imaging and plasmonics

KA Willets, AJ Wilson, V Sundaresan… - Chemical reviews, 2017 - ACS Publications
This review describes the growing partnership between super-resolution imaging and
plasmonics, by describing the various ways in which the two topics mutually benefit one …