Uses of viral vectors have thus far eclipsed uses of non-viral vectors for gene therapy
delivery in the clinic. Viral vectors, however, have certain issues involving genome …

ABSTRACT A major advantage of DNA vaccination is the ability to induce both humoral and
cellular immune responses. DNA vaccines are currently used in veterinary medicine, but …

Transposons are mobile genetic elements evolved to execute highly efficient integration of
their genes into the genomes of their host cells. These natural DNA transfer vehicles have …

The widespread clinical implementation of gene therapy requires the ability to stably
integrate genetic information through gene transfer vectors in a safe, effective, and …

Electroporation is a platform technology for drug and gene delivery. When applied to cell in
vitro or tissues in vivo, it leads to an increase in membrane permeability for molecules which …

DNA vaccination is a disruptive technology that offers the promise of a new rapidly deployed
vaccination platform to treat human and animal disease with gene-based materials …

Starting in 1991, the advance of Tyr-recombinases Flp and Cre enabled superior strategies
for the predictable insertion of transgenes into compatible target sites of mammalian cells …

Traditional DNA transduction routes used for the modification of cellular genomes are
subject to unpredictable alterations, as the cell-intrinsic repair machinery may affect both the …

Introduction: This review presents recent developments in the use of nonviral vectors and
transfer technologies in cancer gene therapy. Tremendous progress has been made in …

Molecular medicine has entered a high-tech age that provides curative treatments of
complex genetic diseases through genetically engineered cellular medicinal products. Their …