AID in antibody diversification: there and back again
Activation-Induced cytidine Deaminase (AID) initiates affinity maturation and isotype
switching by deaminating deoxycytidines within immunoglobulin genes, leading to somatic …
switching by deaminating deoxycytidines within immunoglobulin genes, leading to somatic …
Functions and malfunctions of mammalian DNA-cytosine deaminases
SU Siriwardena, K Chen, AS Bhagwat - Chemical reviews, 2016 - ACS Publications
The AID/APOBEC family enzymes convert cytosines in single-stranded DNA to uracils,
causing base substitutions and strand breaks. They are induced by cytokines produced …
causing base substitutions and strand breaks. They are induced by cytokines produced …
DNA stretching induces Cas9 off-target activity
CRISPR/Cas9 is a powerful genome-editing tool, but spurious off-target edits present a
barrier to therapeutic applications. To understand how CRISPR/Cas9 discriminates between …
barrier to therapeutic applications. To understand how CRISPR/Cas9 discriminates between …
DNA-directed termination of RNA polymerase II transcription
Summary RNA polymerase II (RNAPII) transcription involves initiation from a promoter,
transcriptional elongation through the gene, and termination in the terminator region. In …
transcriptional elongation through the gene, and termination in the terminator region. In …
Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease
Abstract The CRISPR–Cas9 RNA-guided endonuclease system allows precise and efficient
modification of complex genomes and is continuously developed to enhance specificity …
modification of complex genomes and is continuously developed to enhance specificity …
Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts
Cellular protein-RNA complexes assemble on nascent transcripts, but methods to observe
transcription and protein binding in real time and at physiological concentrations are not …
transcription and protein binding in real time and at physiological concentrations are not …
AID in chronic lymphocytic leukemia: induction and action during disease progression
The enzyme activation-induced cytidine deaminase (AID) initiates somatic hypermutation
(SHM) and class switch recombination (CSR) of immunoglobulin (Ig) genes, critical actions …
(SHM) and class switch recombination (CSR) of immunoglobulin (Ig) genes, critical actions …
Structural analysis of the activation-induced deoxycytidine deaminase required in immunoglobulin diversification
P Pham, SA Afif, M Shimoda, K Maeda, N Sakaguchi… - DNA repair, 2016 - Elsevier
Activation-induced deoxycytidine deaminase (AID) initiates somatic hypermutation (SHM)
and class-switch recombination (CSR) by deaminating C→ U during transcription of Ig …
and class-switch recombination (CSR) by deaminating C→ U during transcription of Ig …
Cytidine deaminase efficiency of the lentiviral viral restriction factor APOBEC3C correlates with dimerization
Abstract The seven APOBEC3 (A3) enzymes in primates restrict HIV/SIV replication to
differing degrees by deaminating cytosine in viral (−) DNA, which forms promutagenic …
differing degrees by deaminating cytosine in viral (−) DNA, which forms promutagenic …
Biochemical basis of APOBEC3 deoxycytidine deaminase activity on diverse DNA substrates
The Apolipoprotein B mRNA editing complex (APOBEC) family of enzymes contains single-
stranded polynucleotide cytidine deaminases. These enzymes catalyze the deamination of …
stranded polynucleotide cytidine deaminases. These enzymes catalyze the deamination of …