Proteomic challenges: sample preparation techniques for microgram-quantity protein analysis from biological samples
P Feist, AB Hummon - International journal of molecular sciences, 2015 - mdpi.com
Proteins regulate many cellular functions and analyzing the presence and abundance of
proteins in biological samples are central focuses in proteomics. The discovery and …
proteins in biological samples are central focuses in proteomics. The discovery and …
Less label, more free: approaches in label‐free quantitative mass spectrometry
In this review we examine techniques, software, and statistical analyses used in label‐free
quantitative proteomics studies for area under the curve and spectral counting approaches …
quantitative proteomics studies for area under the curve and spectral counting approaches …
High-throughput proteomics
Mass spectrometry (MS)–based high-throughput proteomics is the core technique for large-
scale protein characterization. Due to the extreme complexity of proteomes, sophisticated …
scale protein characterization. Due to the extreme complexity of proteomes, sophisticated …
Sample preparation by easy extraction and digestion (SPEED)-a universal, rapid, and detergent-free protocol for proteomics based on acid extraction
J Doellinger, A Schneider, M Hoeller… - Molecular & Cellular …, 2020 - ASBMB
The main challenge of bottom-up proteomic sample preparation is to extract proteomes in a
manner that enables efficient protein digestion for subsequent mass spectrometric analysis …
manner that enables efficient protein digestion for subsequent mass spectrometric analysis …
Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phenol extraction for crop proteomic analysis
Crop plants contain large amounts of secondary compounds that interfere with protein
extraction and gel-based proteomic analysis. Thus, a protein extraction protocol that can be …
extraction and gel-based proteomic analysis. Thus, a protein extraction protocol that can be …
Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members
In germ cells, Piwi proteins interact with a specific class of small noncoding RNAs, piwi-
interacting RNAs (piRNAs). Together, these form a pathway that represses transposable …
interacting RNAs (piRNAs). Together, these form a pathway that represses transposable …
A review of nanoscale LC-ESI for metabolomics and its potential to enhance the metabolome coverage
AJ Chetwynd, A David - Talanta, 2018 - Elsevier
Liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS) platforms
are widely used to perform high throughput untargeted profiling of biological samples for …
are widely used to perform high throughput untargeted profiling of biological samples for …
Biomarker discovery in mass spectrometry‐based urinary proteomics
Urinary proteomics has become one of the most attractive topics in disease biomarker
discovery. MS‐based proteomic analysis has advanced continuously and emerged as a …
discovery. MS‐based proteomic analysis has advanced continuously and emerged as a …
Mass spectrometry-based methods for identifying oxidized proteins in disease: advances and challenges
I Verrastro, S Pasha, K Tveen Jensen, AR Pitt… - Biomolecules, 2015 - mdpi.com
Many inflammatory diseases have an oxidative aetiology, which leads to oxidative damage
to biomolecules, including proteins. It is now increasingly recognized that oxidative post …
to biomolecules, including proteins. It is now increasingly recognized that oxidative post …
Quantitative proteomic analysis of gingival crevicular fluid in different periodontal conditions
Aim To quantify the proteome composition of the GCF in periodontal health (HH) and in sites
with different clinical conditions in chronic periodontitis (CP) subjects. Material and Methods …
with different clinical conditions in chronic periodontitis (CP) subjects. Material and Methods …