Proteomic challenges: sample preparation techniques for microgram-quantity protein analysis from biological samples

P Feist, AB Hummon - International journal of molecular sciences, 2015 - mdpi.com
Proteins regulate many cellular functions and analyzing the presence and abundance of
proteins in biological samples are central focuses in proteomics. The discovery and …

Less label, more free: approaches in label‐free quantitative mass spectrometry

KA Neilson, NA Ali, S Muralidharan, M Mirzaei… - …, 2011 - Wiley Online Library
In this review we examine techniques, software, and statistical analyses used in label‐free
quantitative proteomics studies for area under the curve and spectral counting approaches …

High-throughput proteomics

Z Zhang, S Wu, DL Stenoien… - Annual review of …, 2014 - annualreviews.org
Mass spectrometry (MS)–based high-throughput proteomics is the core technique for large-
scale protein characterization. Due to the extreme complexity of proteomes, sophisticated …

Sample preparation by easy extraction and digestion (SPEED)-a universal, rapid, and detergent-free protocol for proteomics based on acid extraction

J Doellinger, A Schneider, M Hoeller… - Molecular & Cellular …, 2020 - ASBMB
The main challenge of bottom-up proteomic sample preparation is to extract proteomes in a
manner that enables efficient protein digestion for subsequent mass spectrometric analysis …

Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phenol extraction for crop proteomic analysis

X Wu, E Xiong, W Wang, M Scali, M Cresti - Nature protocols, 2014 - nature.com
Crop plants contain large amounts of secondary compounds that interfere with protein
extraction and gel-based proteomic analysis. Thus, a protein extraction protocol that can be …

Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members

VV Vagin, J Wohlschlegel, J Qu, Z Jonsson… - Genes & …, 2009 - genesdev.cshlp.org
In germ cells, Piwi proteins interact with a specific class of small noncoding RNAs, piwi-
interacting RNAs (piRNAs). Together, these form a pathway that represses transposable …

A review of nanoscale LC-ESI for metabolomics and its potential to enhance the metabolome coverage

AJ Chetwynd, A David - Talanta, 2018 - Elsevier
Liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS) platforms
are widely used to perform high throughput untargeted profiling of biological samples for …

Biomarker discovery in mass spectrometry‐based urinary proteomics

S Thomas, L Hao, WA Ricke, L Li - PROTEOMICS–Clinical …, 2016 - Wiley Online Library
Urinary proteomics has become one of the most attractive topics in disease biomarker
discovery. MS‐based proteomic analysis has advanced continuously and emerged as a …

Mass spectrometry-based methods for identifying oxidized proteins in disease: advances and challenges

I Verrastro, S Pasha, K Tveen Jensen, AR Pitt… - Biomolecules, 2015 - mdpi.com
Many inflammatory diseases have an oxidative aetiology, which leads to oxidative damage
to biomolecules, including proteins. It is now increasingly recognized that oxidative post …

Quantitative proteomic analysis of gingival crevicular fluid in different periodontal conditions

CM Silva-Boghossian, APV Colombo, M Tanaka… - PloS one, 2013 - journals.plos.org
Aim To quantify the proteome composition of the GCF in periodontal health (HH) and in sites
with different clinical conditions in chronic periodontitis (CP) subjects. Material and Methods …