Influence of DNA extraction methods, PCR inhibitors and quantification methods on real-time PCR assay of biotechnology-derived traits

T Demeke, GR Jenkins - Analytical and bioanalytical chemistry, 2010 - Springer
Biotechnology-derived varieties of canola, cotton, corn and soybean are being grown in the
USA, Canada and other predominantly grain exporting countries. Although the amount of …

Effect of food processing on plant DNA degradation and PCR-based GMO analysis: a review

N Gryson - Analytical and bioanalytical chemistry, 2010 - Springer
The applicability of a DNA-based method for GMO detection and quantification depends on
the quality and quantity of the DNA. Important food-processing conditions, for example …

Detection limits of quantitative and digital PCR assays and their influence in presence–absence surveys of environmental DNA

ME Hunter, RM Dorazio, JSS Butterfield… - Molecular ecology …, 2017 - Wiley Online Library
A set of universal guidelines is needed to determine the limit of detection (LOD) in PCR‐
based analyses of low‐concentration DNA. In particular, environmental DNA (eDNA) studies …

[HTML][HTML] Critical points of DNA quantification by real-time PCR–effects of DNA extraction method and sample matrix on quantification of genetically modified organisms

K Cankar, D Štebih, T Dreo, J Žel, K Gruden - Bmc Biotechnology, 2006 - Springer
Background Real-time PCR is the technique of choice for nucleic acid quantification. In the
field of detection of genetically modified organisms (GMOs) quantification of biotech …

[HTML][HTML] Routes to improving the reliability of low level DNA analysis using real-time PCR

SLR Ellison, CA English, MJ Burns, JT Keer - BMC biotechnology, 2006 - Springer
Background Accurate quantification of DNA using quantitative real-time PCR at low levels is
increasingly important for clinical, environmental and forensic applications. At low …

Absence of detectable transgenes in local landraces of maize in Oaxaca, Mexico (2003–2004)

S Ortiz-García, E Ezcurra, B Schoel… - Proceedings of the …, 2005 - National Acad Sciences
In 2000, transgenes were detected in local maize varieties (landraces) in the mountains of
Oaxaca, Mexico [Quist, D. & Chapela, IH (2001) Nature 414, 541–543]. This region is part of …

Development of three real-time PCR assays to detect peanut allergen residue in processed food products

E Scaravelli, M Brohée, R Marchelli… - … Food Research and …, 2008 - Springer
Hypersensitivity to peanut is a public health problem, since the ingestion of even low
amounts of peanut can trigger severe allergic reactions. Allergic consumers rely on the …

GMO quantification: valuable experience and insights for the future

M Milavec, D Dobnik, L Yang, D Zhang… - Analytical and …, 2014 - Springer
Cultivation and marketing of genetically modified organisms (GMOs) have been unevenly
adopted worldwide. To facilitate international trade and to provide information to consumers …

Immunoassay as an analytical tool in agricultural biotechnology

GD Grothaus, M Bandla, T Currier… - Journal of AOAC …, 2006 - academic.oup.com
Immunoassays for biotechnology engineered proteins are used by AgBiotech companies at
numerous points in product development and by feed and food suppliers for compliance and …

Point of care diagnosis of plant virus: current trends and prospects

K Kalimuthu, J Arivalagan, M Mohan… - Molecular and Cellular …, 2022 - Elsevier
Plant viral diseases account s for major global economic losses in modern-day agriculture.
Plant viral disease management is the primary challenge for both farmers and researchers …