Mechanisms and pathology of protein misfolding and aggregation

N Louros, J Schymkowitz, F Rousseau - Nature Reviews Molecular Cell …, 2023 - nature.com
Despite advances in machine learning-based protein structure prediction, we are still far
from fully understanding how proteins fold into their native conformation. The conventional …

[HTML][HTML] Bridging structural and cell biology with cryo-electron microscopy

E Nogales, J Mahamid - Nature, 2024 - nature.com
Most life scientists would agree that understanding how cellular processes work requires
structural knowledge about the macromolecules involved. For example, deciphering the …

Aggrephagy at a glance

B Bauer, S Martens, L Ferrari - Journal of Cell Science, 2023 - journals.biologists.com
Cells keep their proteome functional by the action of the proteostasis network, composed of
the chaperones, the ubiquitin-proteasome system and autophagy. The decline of this …

Structural basis of plp2-mediated cytoskeletal protein folding by TRiC/CCT

W Han, M Jin, C Liu, Q Zhao, S Wang, Y Wang… - Science …, 2023 - science.org
The cytoskeletal proteins tubulin and actin are the obligate substrates of TCP-1 ring
complex/Chaperonin containing TCP-1 (TRiC/CCT), and their folding involves co …

STYXL1 regulates CCT complex assembly and flagellar tubulin folding in sperm formation

Y Chen, M Luo, H Tu, Y Qi, Y Guo, X Zhang… - Nature …, 2024 - nature.com
Tubulin-based microtubule is a core component of flagella axoneme and essential for sperm
motility and male fertility. Structural components of the axoneme have been well explored …

Brain malformations and seizures by impaired chaperonin function of TRiC

F Kraft, P Rodriguez-Aliaga, W Yuan, L Franken, K Zajt… - Science, 2024 - science.org
Malformations of the brain are common and vary in severity, from negligible to potentially
fatal. Their causes have not been fully elucidated. Here, we report pathogenic variants in the …

Pathway and mechanism of tubulin folding mediated by TRiC/CCT along its ATPase cycle revealed using cryo-EM

C Liu, M Jin, S Wang, W Han, Q Zhao, Y Wang… - Communications …, 2023 - nature.com
The eukaryotic chaperonin TRiC/CCT assists the folding of about 10% of cytosolic proteins
through an ATP-driven conformational cycle, and the essential cytoskeleton protein tubulin …

A hierarchical assembly pathway directs the unique subunit arrangement of TRiC/CCT

KB Moreira, MP Collier, A Leitner, KH Li, ILS Lachapel… - Molecular Cell, 2023 - cell.com
How the essential eukaryotic chaperonin TRiC/CCT assembles from eight distinct subunits
into a unique double-ring architecture remains undefined. We show TRiC assembly involves …

In silico protein dynamics in the human cytoplasm: Partial folding, misfolding, fold switching, and non‐native interactions

PPS Russell, MM Rickard, M Boob, M Gruebele… - Protein …, 2023 - Wiley Online Library
We examine the influence of cellular interactions in all‐atom models of a section of the
Homo sapiens cytoplasm on the early folding events of the three‐helix bundle protein B …

Mechanistic insights into protein folding by the eukaryotic chaperonin complex CCT

TM Smith, BM Willardson - Biochemical Society Transactions, 2022 - portlandpress.com
The cytosolic chaperonin CCT is indispensable to eukaryotic life, folding the cytoskeletal
proteins actin and tubulin along with an estimated 10% of the remaining proteome …