Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) imaging mass
spectrometry (IMS) is an evolving technique in cancer diagnostics and combines the …

Oncoproteomics is the study of proteins and their interactions in a cancer cell by proteomic
technologies. Proteomic research first came to the fore with the introduction of two …

Electron transfer dissociation (ETD) is a recently introduced mass spectrometric technique
that provides a more comprehensive coverage of peptide sequences and posttranslational …

A novel method for high‐throughput proteomic analysis of formalin‐fixed paraffin‐embedded
(FFPE) tissue microarrays (TMA) is described using on‐tissue tryptic digestion followed by …

Formalin fixation, generally followed by paraffin embedding, is the standard and well-
established processing method employed by pathologist. This treatment conserves and …

Tissue samples in biobanks are typically formalin-fixed and paraffin-embedded (FFPE), in
which form they are preserved for decades. It has only recently been shown that proteins in …

Retrospective proteomic studies, including those which aim to elucidate the molecular
mechanisms driving cancer, require the assembly and characterization of substantial patient …

A satisfactory protocol of protein extraction has been established based on the heat-induced
antigen retrieval (AR) technique widely applied in immunohistochemistry for archival …

Imaging MS is a powerful technique that combines the chemical and spatial analysis of
surfaces. It allows spatial localization of multiple different compounds that are recorded in …

Formalin-fixed paraffin-embedded (FFPE) tissue specimens comprise a potentially valuable
resource for retrospective biomarker discovery studies, and recent work indicates the …