Resequencing and mutational analysis using oligonucleotide microarrays

JG Hacia - Nature genetics, 1999 - nature.com
Oligonucleotide microarray (DNA chip)–based hybridization analysis is a promising new
technology which potentially allows rapid and cost–effective screens for all possible …

Survey and summary: The applications of universal DNA base analogues

D Loakes - Nucleic Acids Research, 2001 - academic.oup.com
A universal base analogue forms 'base pairs' with each of the natural DNA/RNA bases with
little discrimination between them. A number of such analogues have been prepared and …

In situ localized amplification and contact replication of many individual DNA molecules

RD Mitra, GM Church - Nucleic Acids Research, 1999 - academic.oup.com
We describe a method to clone and amplify DNA by performing the polymerase chain
reaction (PCR) in a thin polyacrylamide film poured on a glass microscope slide. The …

Signal detection techniques for the detection of analytes

HH Duong, SD O'connor, RH Terbrueggen… - US Patent …, 2004 - Google Patents
(73) Assignee: Clinical Micro Sensors, Inc., Pasadena, 5,601,982 A 2/1997 Sargent et
al.......... 435/6 CA (US) 5,620,850 A 4/1997 Bamdad et al.............. 530/300 5,622,821 A …

[HTML][HTML] Universal DNA microarray method for multiplex detection of low abundance point mutations

NP Gerry, NE Witowski, J Day, RP Hammer… - Journal of molecular …, 1999 - Elsevier
Cancers arise from the accumulation of multiple mutations in genes regulating cellular
growth and differentiation. Identification of such mutations in numerous genes represents a …

Nearest-Neighbor Thermodynamics of Internal A⊙ C Mismatches in DNA: Sequence Dependence and pH Effects

HT Allawi, J SantaLucia - Biochemistry, 1998 - ACS Publications
Thermodynamics of 27 oligonucleotides with internal A⊙ C mismatches at two different pHs
were determined from UV absorbance versus temperature melting profiles. The data were …

Devices and methods for biochip multiplexing

G Blackburn, HH Duong, P Grodzinski… - US Patent …, 2007 - Google Patents
2002-07-15 Assigned to CLINICAL MICRO SENSORS, INC. reassignment CLINICAL
MICRO SENSORS, INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT …

Direct detection of 16S rRNA in soil extracts by using oligonucleotide microarrays

J Small, DR Call, FJ Brockman, TM Straub… - Applied and …, 2001 - Am Soc Microbiol
We report on the development and validation of a simple microarray method for the direct
detection of intact 16S rRNA from unpurified soil extracts. Total RNAs from Geobacter …

Microfluidic systems in the electrochemical detection of target analytes

JF Kayyem - US Patent 6,942,771, 2005 - Google Patents
US6942771B1 - Microfluidic systems in the electrochemical detection of target analytes -
Google Patents US6942771B1 - Microfluidic systems in the electrochemical detection of target …

Manual manufacturing of oligonucleotide, DNA, and protein microchips

D Guschin, G Yershov, A Zaslavsky, A Gemmell… - Analytical …, 1997 - Elsevier
A simple procedure for manufacturing microchips containing various gel-immobilized
compounds is described. A gel photopolymerization technique is introduced to produce …