Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

Cryo-electron microscopy (cryo-EM) is rapidly becoming an attractive method in the field of
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …

Native mass spectrometry (MS) has become widely accepted in structural biology, providing
information on stoichiometry, interactions, homogeneity, and shape of protein complexes …

This review summarizes the current state of methods and results achievable by cryo-electron
microscopy (cryo-EM) imaging for molecular, cell, and structural biologists who wish to …

Membrane proteins are highly diverse in both structure and function and can, therefore,
present different challenges for structure determination. They are biologically important for …

Recognizing that interaction with the air–water interface (AWI) is a major challenge for cryo-
EM, we first review current approaches designed to avoid it. Of these, immobilizing particles …

Nanobodies are popular and versatile tools for structural biology. They have a compact
single immunoglobulin domain organization, bind target proteins with high affinities while …

Molecular self-assembly is pervasive in the formation of living and synthetic materials.
Knowledge gained from research into the principles of molecular self-assembly drives …

Mechanistic understanding of biochemical reactions requires structural and kinetic
characterization of the underlying chemical processes. However, no single experimental …

Cryogenic electron microscopy (cryo-EM) has become one of the most powerful techniques
to reveal the atomic structures and working mechanisms of biological macromolecules. New …