Based on engineered or bacterial nucleases, the development of genome editing
technologies has opened up the possibility of directly targeting and modifying genomic …

A synthetic lethal interaction occurs between two genes when the perturbation of either gene
alone is viable but the perturbation of both genes simultaneously results in the loss of …

The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …

CRISPR/Cas9 has revolutionized our ability to engineer genomes and conduct genome-
wide screens in human cells,–. Whereas some cell types are amenable to genome …

Most human epithelial tumors harbor numerous alterations, making it difficult to predict
which genes are required for tumor survival. To systematically identify cancer dependencies …

Elucidation of the mutational landscape of human cancer has progressed rapidly and been
accompanied by the development of therapeutics targeting mutant oncogenes. However, a …

Forward genetic screens are powerful tools for the unbiased discovery and functional
characterization of specific genetic elements associated with a phenotype of interest …

The CRISPR–Cas9 system has revolutionized gene editing both at single genes and in
multiplexed loss-of-function screens, thus enabling precise genome-scale identification of …

We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA. Here,
we build on this observation with a comprehensive algorithm that incorporates chromatin …

The genetic dependencies of human cancers widely vary. Here, we catalog this
heterogeneity and use it to identify functional gene interactions and genotype-dependent …