In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

In-cell NMR spectroscopy provides precious structural and functional information on
biological macromolecules in their native cellular environment at atomic resolution …

In-cell NMR can investigate protein conformational changes at atomic resolution, such as
those changes induced by drug binding or chemical modifications, directly in living human …

One of the greatest current challenges in structural biology is to study protein dynamics over
a wide range of timescales in complex environments, such as the cell. Among magnetic …

Highlights•NMR is most suitable for the structural characterization of glycoproteins.•Protein
overexpression in eukaryotic media can produce glycoproteins at high yield and with …

Protein phosphorylation is an abundant post-translational modification (PTM) and an
essential modulator of protein functionality in living cells. Intrinsically disordered proteins …

Natural product-observed NMR methods have considerably expanded the potentialities for
in-tube NMR monitoring of complex enzymatic biotransformations and investigation of …

Extracellular matrix glycoproteins play a major role in bone mineralization and modulation of
osteogenesis. Among these, the intrinsically disordered protein osteopontin (OPN) is …

The determination of the binding affinity quantifying the interaction between proteins and
nucleic acids is of crucial interest in biological and chemical research. Here, we have made …