The molecular basis for cellular function of intrinsically disordered protein regions

AS Holehouse, BB Kragelund - Nature Reviews Molecular Cell Biology, 2024 - nature.com
Intrinsically disordered protein regions exist in a collection of dynamic interconverting
conformations that lack a stable 3D structure. These regions are structurally heterogeneous …

Biomolecular condensates in the nucleus

BR Sabari, A Dall'Agnese, RA Young - Trends in biochemical sciences, 2020 - cell.com
Nuclear processes such as DNA replication, transcription, and RNA processing each
depend on the concerted action of many different protein and RNA molecules. How …

Aberrant phase separation and nucleolar dysfunction in rare genetic diseases

MA Mensah, H Niskanen, AP Magalhaes, S Basu… - Nature, 2023 - nature.com
Thousands of genetic variants in protein-coding genes have been linked to disease.
However, the functional impact of most variants is unknown as they occur within intrinsically …

Sequence-dependent surface condensation of a pioneer transcription factor on DNA

JA Morin, S Wittmann, S Choubey, A Klosin, S Golfier… - Nature Physics, 2022 - nature.com
Biomolecular condensates are dense assemblies of proteins that form distinct biochemical
compartments without being surrounded by a membrane. Some, such as P granules and …

Identifying synergistic high-order 3D chromatin conformations from genome-scale nanopore concatemer sequencing

AS Deshpande, N Ulahannan, M Pendleton… - Nature …, 2022 - nature.com
Abstract High-order three-dimensional (3D) interactions between more than two genomic
loci are common in human chromatin, but their role in gene regulation is unclear. Previous …

The road ahead in genetics and genomics

AL McGuire, S Gabriel, SA Tishkoff, A Wonkam… - Nature Reviews …, 2020 - nature.com
In celebration of the 20th anniversary of Nature Reviews Genetics, we asked 12 leading
researchers to reflect on the key challenges and opportunities faced by the field of genetics …

Phase separation as a missing mechanism for interpretation of disease mutations

B Tsang, I Pritišanac, SW Scherer, AM Moses… - Cell, 2020 - cell.com
It is unclear how disease mutations impact intrinsically disordered protein regions (IDRs),
which lack a stable folded structure. These mutations, while prevalent in disease, are …

Sequence-encoded and composition-dependent protein-RNA interactions control multiphasic condensate morphologies

T Kaur, M Raju, I Alshareedah, RB Davis… - Nature …, 2021 - nature.com
Multivalent protein-protein and protein-RNA interactions are the drivers of biological phase
separation. Biomolecular condensates typically contain a dense network of multiple proteins …

Liquid–liquid phase separation in chromatin

K Rippe - Cold Spring Harbor perspectives in biology, 2022 - cshperspectives.cshlp.org
In eukaryotic cells, protein and RNA factors involved in genome activities like transcription,
RNA processing, DNA replication, and repair accumulate in self-organizing membraneless …

Genetic variation associated with condensate dysregulation in disease

SF Banani, LK Afeyan, SW Hawken, JE Henninger… - Developmental cell, 2022 - cell.com
A multitude of cellular processes involve biomolecular condensates, which has led to the
suggestion that diverse pathogenic mutations may dysregulate condensates. Although proof …