The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …

Enhanced agricultural production through innovative breeding technology is urgently
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …

Targeted DNA double-strand breaks have been shown to significantly increase the
frequency and precision of genome editing. In the past two decades, several double-strand …

Targeted mutagenesis, editing of endogenous maize (Zea mays) genes, and site-specific
insertion of a trait gene using clustered regularly interspaced short palindromic repeats …

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9
(Cas9) genome editing system is a powerful tool for targeted gene modifications in a wide …

There is a wide range of application for nanotechnology in agriculture, including fertilizers,
aquaculture, irrigation, water filtration, animal feed, animal vaccines, food processing, and …

The ability to edit plant genomes through gene targeting (GT) requires efficient methods to
deliver both sequence‐specific nucleases (SSN s) and repair templates to plant cells. This is …

In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a
homologous DNA sequence (ie, homologous recombination [HR]), focusing mainly on yeast …

Agriculture is an important source of human food. However, current agricultural practices
need modernizing and strengthening to fulfill the increasing food requirements of the …

Reverse genetics in model organisms such as Drosophila melanogaster, Arabidopsis
thaliana, zebrafish and rats, efficient genome engineering in human embryonic stem and …