Artificial biocatalytic linear cascades for preparation of organic molecules

JH Schrittwieser, S Velikogne, M Hall… - Chemical …, 2018 - ACS Publications
The review compiles artificial cascades involving enzymes with a focus on the last 10 years.
A cascade is defined as the combination of at least two reaction steps in a single reaction …

Hydrogen peroxide driven biocatalysis

BO Burek, S Bormann, F Hollmann, JZ Bloh… - Green …, 2019 - pubs.rsc.org
In general, hydrogen peroxide is a stable and relatively mild oxidant and it can be regarded
as the ultimate “green” reagent because water and oxygen are the only by-products …

Hydrocarbon synthesis via photoenzymatic decarboxylation of carboxylic acids

W Zhang, M Ma, MME Huijbers… - Journal of the …, 2019 - ACS Publications
A recently discovered photodecarboxylase from Chlorella variabilis NC64A (Cv FAP) bears
the promise for the efficient and selective synthesis of hydrocarbons from carboxylic acids …

Photobiocatalytic synthesis of chiral secondary fatty alcohols from renewable unsaturated fatty acids

W Zhang, JH Lee, SHH Younes, F Tonin… - Nature …, 2020 - nature.com
En route to a bio-based chemical industry, the conversion of fatty acids into building blocks
is of particular interest. Enzymatic routes, occurring under mild conditions and excelling by …

Mechanistic studies of fatty acid activation by CYP152 peroxygenases reveal unexpected desaturase activity

M Pickl, S Kurakin, FG Cantú Reinhard, P Schmid… - ACS …, 2018 - ACS Publications
The majority of cytochrome P450 enzymes (CYPs) predominantly operate as
monooxygenases, but recently a class of P450 enzymes was discovered, that can act as …

Chemo-enzymatic cascades to produce cycloalkenes from bio-based resources

S Wu, Y Zhou, D Gerngross, M Jeschek… - Nature …, 2019 - nature.com
Engineered enzyme cascades offer powerful tools to convert renewable resources into
value-added products. Man-made catalysts give access to new-to-nature reactivities that …

Regioselective biocatalytic hydroxylation of fatty acids by cytochrome P450s

L Hammerer, CK Winkler, W Kroutil - Catalysis Letters, 2018 - Springer
Cytochrome P450 enzymes catalyze a broad set of C–H activation reactions, the most
prominent being hydroxylation. This review provides an overview of the regioselectivity (CH …

The enigmatic P450 decarboxylase OleT is capable of, but evolved to frustrate, oxygen rebound chemistry

CH Hsieh, X Huang, JA Amaya, CD Rutland… - Biochemistry, 2017 - ACS Publications
OleT is a cytochrome P450 enzyme that catalyzes the removal of carbon dioxide from
variable chain length fatty acids to form 1-alkenes. In this work, we examine the binding and …

[HTML][HTML] Biosynthesis of organic molecules via artificial cascade reactions based on cytochrome P450 monooxygenases

RJ Li, Z Zhang, CG Acevedo-Rocha, J Zhao… - Green Synthesis and …, 2020 - Elsevier
Cytochrome P450 monooxygenases (P450s) play crucial roles in the oxyfunctionalization of
non-activated hydrocarbons, thus bridging the gap between simple molecules and high …

An engineered self-sufficient biocatalyst enables scalable production of linear α-olefins from carboxylic acids

C Lu, F Shen, S Wang, Y Wang, J Liu, WJ Bai… - ACS …, 2018 - ACS Publications
Fusing the decarboxylase OleTJE and the reductase domain of P450BM3 creates a self-
sufficient protein, OleT-BM3R, which is able to efficiently catalyze oxidative decarboxylation …