Redefining fundamental concepts of transcription initiation in bacteria

C Mejía-Almonte, SJW Busby, JT Wade… - Nature Reviews …, 2020 - nature.com
Despite enormous progress in understanding the fundamentals of bacterial gene regulation,
our knowledge remains limited when compared with the number of bacterial genomes and …

CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications

DA Burbano, C Kiattisewee, AV Karanjia… - Annual Review of …, 2024 - annualreviews.org
In the past decades, the broad selection of CRISPR-Cas systems has revolutionized
biotechnology by enabling multimodal genetic manipulation in diverse organisms. Rooted in …

Trees, fungi and bacteria: tripartite metatranscriptomics of a root microbiome responding to soil contamination

E Gonzalez, FE Pitre, AP Pagé, J Marleau… - Microbiome, 2018 - Springer
Background One method for rejuvenating land polluted with anthropogenic contaminants is
through phytoremediation, the reclamation of land through the cultivation of specific crops …

The Transcription Unit Architecture of Streptomyces lividans TK24

Y Lee, N Lee, Y Jeong, S Hwang, W Kim… - Frontiers in …, 2019 - frontiersin.org
Streptomyces lividans is an attractive host for production of heterologous proteins and
secondary metabolites of other Streptomyces species. To fully harness the industrial …

The Transcriptional landscape of Streptococcus pneumoniae TIGR4 reveals a complex operon architecture and abundant riboregulation critical for growth and …

I Warrier, N Ram-Mohan, Z Zhu, A Hazery… - PLoS …, 2018 - journals.plos.org
Efficient and highly organized regulation of transcription is fundamental to an organism's
ability to survive, proliferate, and quickly respond to its environment. Therefore, precise …

Mining Novel Constitutive Promoter Elements in Soil Metagenomic Libraries in Escherichia coli

CA Westmann, LF Alves, R Silva-Rocha… - Frontiers in …, 2018 - frontiersin.org
Although functional metagenomics has been widely employed for the discovery of genes
relevant to biotechnology and biomedicine, its potential for assessing the diversity of …

Transcriptomics-driven characterization of LUZ100, a T7-like Pseudomonas phage with temperate features

L Putzeys, J Poppeliers, M Boon, C Lood, M Vallino… - Msystems, 2023 - Am Soc Microbiol
Autographiviridae is a diverse yet distinct family of bacterial viruses marked by a strictly lytic
lifestyle and a generally conserved genome organization. Here, we characterized …

Genome-scale analysis of genetic regulatory elements in Streptomyces avermitilis MA-4680 using transcript boundary information

Y Lee, N Lee, S Hwang, W Kim, S Cho, BO Palsson… - BMC genomics, 2022 - Springer
Background The gram-positive bacterium, Streptomyces avermitilis, holds industrial
importance as the producer of avermectin, a widely used anthelmintic agent, and a …

Cross-evaluation of E. coli's operon structures via a whole-cell model suggests alternative cellular benefits for low-versus high-expressing operons

G Sun, MM DeFelice, TE Gillies, TA Ahn-Horst… - Cell Systems, 2024 - cell.com
Many bacteria use operons to coregulate genes, but it remains unclear how operons benefit
bacteria. We integrated E. coli's 788 polycistronic operons and 1,231 transcription units into …

BAC-BROWSER: the tool for visualization and analysis of prokaryotic genomes

IA Garanina, GY Fisunov, VM Govorun - Frontiers in microbiology, 2018 - frontiersin.org
Prokaryotes are actively studied objects in the scope of genomic regulation. Microbiologists
need special tools for complex analysis of data to study and identification of regulatory …