Point mutations cause members of the serine protease inhibitor (serpin) superfamily to
undergo a novel conformational transition, forming ordered polymers. These polymers …

Osmolytes are naturally occurring organic compounds, which represent different chemical
classes including amino acids, methylamines, and polyols. By accumulating high …

The molecular basis of α1-antitrypsin deficiency is reviewed and is shown to be due to the
accumulation of mutant protein as ordered polymers within the endoplasmic reticulum of …

Osmolytes are naturally occurring small molecules accumulated intracellularly to protect
organisms from various denaturing stresses. Similar to the two faces of a coin, several of …

Many studies aimed at advancing our understanding of the mechanisms of protein
stabilization that support the survival of organisms under harsh environmental conditions are …

Since protein function depends on folding, successful development of active pharmaceutical
proteins requires in vitro production of functional, properly folded proteins. In vitro protein …

Most of the neurological disorders in the brain are caused by the abnormal buildup of
misfolded or aggregated proteins. Osmolytes are low molecular weight organic molecules …

Abstract Background α1-antitrypsin (AAT) serves primarily as an inhibitor of the elastin
degrading proteases, neutrophil elastase and proteinase 3. There is ample clinical evidence …

T4 DNA ligase is capable of ligating 2′ OMe-RNA duplexes, HNA, LNA and FANA mixed
sequences in the presence of 10% w/v PEG8000 and 3 M betaine. The enzymatic joining of …

Expression systems based on Escherichia coli offer fast, cheap, and convenient means for
the production of recombinant serpins. Over 30 active serpins from prokaryotic and …