The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence

J Kopeika, A Thornhill, Y Khalaf - Human reproduction update, 2015 - academic.oup.com
BACKGROUND Cryopreservation has been extensively used in assisted reproductive
technology, agriculture and conservation programmes for endangered species. The …

Cryopreservation of fish sperm: applications and perspectives

E Cabrita, C Sarasquete… - Journal of Applied …, 2010 - Wiley Online Library
Cryopreservation is of interest not only for fish farming but also for the conservation and
genetic improvement of resources. This technique has been well established in some …

Gamete quality and broodstock management in temperate fish

H Migaud, G Bell, E Cabrita, B McAndrew… - Reviews in …, 2013 - Wiley Online Library
The ability to fully control sexual maturation and spawning and to produce large numbers of
high quality seeds 'on demand'(ie all year long) is a primary requirement for the successful …

Effects of Glyphosate and its Formulation, Roundup, on Reproduction in Zebrafish (Danio rerio)

TM Uren Webster, LV Laing, H Florance… - … Science & Technology, 2014 - ACS Publications
Roundup and its active ingredient glyphosate are among the most widely used herbicides
worldwide and may contaminate surface waters. Research suggests both Roundup and …

Factors enhancing fish sperm quality and emerging tools for sperm analysis

E Cabrita, S Martínez-Páramo, PJ Gavaia, MF Riesco… - Aquaculture, 2014 - Elsevier
With this review we try to give a comprehensive overview of the current methods used in
research to assess sperm quality. In addition, we identify some of the most important factors …

The Comet Assay and its applications in the field of ecotoxicology: a mature tool that continues to expand its perspectives

J de Lapuente, J Lourenço, SA Mendo, M Borràs… - Frontiers in …, 2015 - frontiersin.org
Since Singh and colleagues, in 1988, launched to the scientific community the alkaline
Single Cell Gel Electrophoresis (SCGE) protocol, or Comet Assay, its uses and applications …

The influence of certain aminoacids and vitamins on post-thaw fish sperm motility, viability and DNA fragmentation

E Cabrita, S Ma, P Diogo, S Martínez-Páramo… - Animal reproduction …, 2011 - Elsevier
During cryopreservation, dilution in the extender media reduces the seminal plasma
constituents being cells more vulnerable to oxidative stress. Vitamins (C and E) and the …

Effects of short‐term storage on sperm function in fish semen: a review

P Contreras, K Dumorné… - Reviews in …, 2020 - Wiley Online Library
Storage of refrigerated semen is a simple and inexpensive procedure that can facilitate the
management and reproduction programmes in aquaculture and allows to store semen for in …

Fish sperm motility assessment as a tool for aquaculture research: a historical approach

V Gallego, JF Asturiano - Reviews in Aquaculture, 2019 - Wiley Online Library
Fish sperm motility is nowadays considered the best biomarker for the quality of fish
spermatozoa, and sperm motion parameters from more than 300 fish species have been …

Oxidative stress and use of antioxidants in fish semen cryopreservation

L Sandoval‐Vargas, M Silva Jimenez… - Reviews in …, 2021 - Wiley Online Library
Reactive oxygen species (ROS) have been proposed as one of the main causes of the
impairment of fish spermatozoa integrity and functionality during cryopreservation. The high …