CRISPR technology: A decade of genome editing is only the beginning
JY Wang, JA Doudna - Science, 2023 - science.org
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …
editing, coupled with advances in computing and imaging capabilities, has initiated a new …
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
CRISPR-CasΦ from huge phages is a hypercompact genome editor
CRISPR-Cas systems are found widely in prokaryotes, where they provide adaptive
immunity against virus infection and plasmid transformation. We describe a minimal …
immunity against virus infection and plasmid transformation. We describe a minimal …
Base editing: advances and therapeutic opportunities
EM Porto, AC Komor, IM Slaymaker… - Nature Reviews Drug …, 2020 - nature.com
Base editing—the introduction of single-nucleotide variants (SNVs) into DNA or RNA in
living cells—is one of the most recent advances in the field of genome editing. As around …
living cells—is one of the most recent advances in the field of genome editing. As around …
CRISPR gene therapy: applications, limitations, and implications for the future
A series of recent discoveries harnessing the adaptive immune system of prokaryotes to
perform targeted genome editing is having a transformative influence across the biological …
perform targeted genome editing is having a transformative influence across the biological …
How to measure and evaluate binding affinities
I Jarmoskaite, I AlSadhan, PP Vaidyanathan… - Elife, 2020 - elifesciences.org
Quantitative measurements of biomolecule associations are central to biological
understanding and are needed to build and test predictive and mechanistic models. Given …
understanding and are needed to build and test predictive and mechanistic models. Given …
A highly sensitive CRISPR‐empowered surface plasmon resonance sensor for diagnosis of inherited diseases with femtomolar‐level real‐time quantification
The clustered regularly interspaced short palindromic repeats (CRISPR) molecular system
has emerged as a promising technology for the detection of nucleic acids. Herein, the …
has emerged as a promising technology for the detection of nucleic acids. Herein, the …
Simultaneous dual‐gene diagnosis of SARS‐CoV‐2 based on CRISPR/Cas9‐mediated lateral flow assay
E Xiong, L Jiang, T Tian, M Hu, H Yue… - Angewandte Chemie …, 2021 - Wiley Online Library
Few methods for the detection of SARS‐CoV‐2 currently have the capability to
simultaneously detect two genes in a single test, which is a key measure to improve …
simultaneously detect two genes in a single test, which is a key measure to improve …
CRISPR-based genome editing through the lens of DNA repair
Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
Lung SORT LNPs enable precise homology-directed repair mediated CRISPR/Cas genome correction in cystic fibrosis models
Abstract Approximately 10% of Cystic Fibrosis (CF) patients, particularly those with CF
transmembrane conductance regulator (CFTR) gene nonsense mutations, lack effective …
transmembrane conductance regulator (CFTR) gene nonsense mutations, lack effective …