Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
Methods for the directed evolution of proteins
MS Packer, DR Liu - Nature Reviews Genetics, 2015 - nature.com
Directed evolution has proved to be an effective strategy for improving or altering the activity
of biomolecules for industrial, research and therapeutic applications. The evolution of …
of biomolecules for industrial, research and therapeutic applications. The evolution of …
Engineering growth factors for regenerative medicine applications
AC Mitchell, PS Briquez, JA Hubbell, JR Cochran - Acta biomaterialia, 2016 - Elsevier
Growth factors are important morphogenetic proteins that instruct cell behavior and guide
tissue repair and renewal. Although their therapeutic potential holds great promise in …
tissue repair and renewal. Although their therapeutic potential holds great promise in …
Aptamers: an emerging class of molecules that rival antibodies in diagnostics
SD Jayasena - Clinical chemistry, 1999 - academic.oup.com
Antibodies, the most popular class of molecules providing molecular recognition needs for a
wide range of applications, have been around for more than three decades. As a result …
wide range of applications, have been around for more than three decades. As a result …
A general strategy for the evolution of bond-forming enzymes using yeast display
The ability to routinely generate efficient protein catalysts of bond-forming reactions chosen
by researchers, rather than nature, is a long-standing goal of the molecular life sciences …
by researchers, rather than nature, is a long-standing goal of the molecular life sciences …
Isolating and engineering human antibodies using yeast surface display
G Chao, WL Lau, BJ Hackel, SL Sazinsky… - Nature protocols, 2006 - nature.com
This protocol describes the process of isolating and engineering antibodies or proteins for
increased affinity and stability using yeast surface display. Single-chain antibody fragments …
increased affinity and stability using yeast surface display. Single-chain antibody fragments …
Compositions and methods for molecular labeling
M Samuels, JC Olson, A Watson, K Brown… - US Patent …, 2015 - Google Patents
2012-05-08 Assigned to RAINDANCE TECHNOLOGIES, INC. reassignment RAINDANCE
TECHNOLOGIES, INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR …
TECHNOLOGIES, INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR …
[HTML][HTML] A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules
LA Banaszynski, L Chen, LA Maynard-Smith, AGL Ooi… - Cell, 2006 - cell.com
Rapid and reversible methods for perturbing the function of specific proteins are desirable
tools for probing complex biological systems. We have developed a general technique to …
tools for probing complex biological systems. We have developed a general technique to …
Tuning genetic control through promoter engineering
Gene function is typically evaluated by sampling the continuum of gene expression at only a
few discrete points corresponding to gene knockout or overexpression. We argue that this …
few discrete points corresponding to gene knockout or overexpression. We argue that this …
Laboratory evolution of stereoselective enzymes: a prolific source of catalysts for asymmetric reactions
MT Reetz - Angewandte Chemie International Edition, 2011 - Wiley Online Library
Asymmetric catalysis plays a key role in modern synthetic organic chemistry, with synthetic
catalysts and enzymes being the two available options. During the latter part of the last …
catalysts and enzymes being the two available options. During the latter part of the last …