Multi-wavelength analytical ultracentrifugation of biopolymer mixtures and interactions

A Henrickson, GE Gorbet, A Savelyev, M Kim… - Analytical …, 2022 - Elsevier
Multi-wavelength analytical ultracentrifugation (MW-AUC) is a recent development made
possible by new analytical ultracentrifuge optical systems. MW-AUC extends the basic …

[HTML][HTML] Moving analytical ultracentrifugation software to a good manufacturing practices (GMP) environment

A Savelyev, GE Gorbet, A Henrickson… - PLoS computational …, 2020 - journals.plos.org
Recent advances in instrumentation have moved analytical ultracentrifugation (AUC) closer
to a possible validation in a Good Manufacturing Practices (GMP) environment. In order for …

A new UltraScan module for the characterization and quantification of analytical buoyant density equilibrium experiments to determine AAV capsid loading

A Savelyev, EH Brookes, A Henrickson… - European Biophysics …, 2023 - Springer
A method for characterizing and quantifying peaks formed in an analytical buoyant density
equilibrium (ABDE) experiment is presented. An algorithm is derived to calculate the …

Suitability of double-stranded DNA as a molecular standard for the validation of analytical ultracentrifugation instruments

M Ranasinghe, JM Fogg, DJ Catanese Jr… - European Biophysics …, 2023 - Springer
To address the current lack of validated molecular standards for analytical ultracentrifugation
(AUC), we investigated the suitability of double-stranded DNA molecules. We compared the …

Methods for the Design and Analysis of Analytical Ultracentrifugation Experiments

B Demeler - Current Protocols, 2024 - Wiley Online Library
Analytical ultracentrifugation experiments play an integral role in the solution‐phase
characterization of biological macromolecules and their interactions. This unit discusses the …

[HTML][HTML] Development of an advanced multiwavelength emission detector for the analytical ultracentrifuge

V Lautenbach, G Onishchukov, SE Wawra… - Nanoscale …, 2024 - pubs.rsc.org
An advanced design of the analytical ultracentrifuge with multiwavelength emission
detection (MWE-AUC) is presented which offers outstanding performance concerning the …

Boundary convection during sedimentation velocity in the Optima analytical ultracentrifuge

SA Berkowitz, T Laue - Analytical Biochemistry, 2021 - Elsevier
Analytical ultracentrifugation (AUC) provides the most widely applicable, precise, and
accurate means for characterizing solution hydrodynamic and thermodynamic properties …

[HTML][HTML] Analytical ultracentrifugation: still the gold standard that offers multiple solutions

RCJ Dobson, TR Patel - European Biophysics Journal, 2020 - Springer
Understanding the nature of macromolecules and their interactions in solution underpins
many fields, including biology, chemistry and materials science. The 24th International …

Calibrating analytical ultracentrifuges

H Zhao, A Nguyen, SC To, P Schuck - European Biophysics Journal, 2021 - Springer
Analytical ultracentrifugation (AUC) is based on the concept of recording and analyzing
macroscopic macromolecular redistribution that results from a centrifugal force acting on the …

Measuring compressibility in the optima AUC™ analytical ultracentrifuge

M Stoutjesdyk, E Brookes, A Henrickson… - European Biophysics …, 2020 - Springer
A method is described to accurately measure the compressibility of liquids using an
analytical ultracentrifuge. The method makes use of very large pressure gradients, which …