Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Metalloenzymes catalyze a variety of reactions using a limited number of natural amino
acids and metallocofactors. Therefore, the environment beyond the primary coordination …

The efficiency, selectivity and sustainability benefits offered by enzymes are enticing
chemists to consider biocatalytic transformations to complement or even supplant more …

Naturally occurring enzymes can be a source of unnatural reactivity that can be molded by
directed evolution to generate efficient biocatalysts with valuable activities. Owing to the lack …

To reduce experimental effort associated with directed protein evolution and to explore the
sequence space encoded by mutating multiple positions simultaneously, we incorporate …

I am a chemist and engineer who looks upon the living world with the deepest admiration.
Nature, herself a brilliant chemist and by far the best engineer of all time, invented life that …

The effective design of combinatorial libraries to balance fitness and diversity facilitates the
engineering of useful enzyme functions, particularly those that are poorly characterized or …

The ability of one enzyme to catalyse multiple, mechanistically distinct transformations likely
played a crucial role in organisms' abilities to adapt to changing external stimuli in the past …

Artificial metalloenzymes (ArMs) result from the incorporation of an abiotic metal cofactor
within a protein scaffold. From the earliest techniques of transition metals adsorbed on silk …

Discovered as organometallic curiosities in the 1970s, carbene radicals have become a
staple in modern-day homogeneous catalysis. Carbene radicals exhibit nucleophilic radical …