Quantitative, high-resolution proteomics for data-driven systems biology
Systems biology requires comprehensive data at all molecular levels. Mass spectrometry
(MS)-based proteomics has emerged as a powerful and universal method for the global …
(MS)-based proteomics has emerged as a powerful and universal method for the global …
Proteome-scale human interactomics
Cellular functions are mediated by complex interactome networks of physical, biochemical,
and functional interactions between DNA sequences, RNA molecules, proteins, lipids, and …
and functional interactions between DNA sequences, RNA molecules, proteins, lipids, and …
The BioPlex network: a systematic exploration of the human interactome
EL Huttlin, L Ting, RJ Bruckner, F Gebreab, MP Gygi… - Cell, 2015 - cell.com
Protein interactions form a network whose structure drives cellular function and whose
organization informs biological inquiry. Using high-throughput affinity-purification mass …
organization informs biological inquiry. Using high-throughput affinity-purification mass …
[HTML][HTML] Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways
Summary Nephronophthisis (NPHP), Joubert (JBTS), and Meckel-Gruber (MKS) syndromes
are autosomal-recessive ciliopathies presenting with cystic kidneys, retinal degeneration …
are autosomal-recessive ciliopathies presenting with cystic kidneys, retinal degeneration …
Centrosomes are autocatalytic droplets of pericentriolar material organized by centrioles
D Zwicker, M Decker, S Jaensch… - Proceedings of the …, 2014 - National Acad Sciences
Centrosomes are highly dynamic, spherical organelles without a membrane. Their physical
nature and their assembly are not understood. Using the concept of phase separation, we …
nature and their assembly are not understood. Using the concept of phase separation, we …
Regulated assembly of a supramolecular centrosome scaffold in vitro
JB Woodruff, O Wueseke, V Viscardi, J Mahamid… - Science, 2015 - science.org
The centrosome organizes microtubule arrays within animal cells and comprises two
centrioles surrounded by an amorphous protein mass called the pericentriolar material …
centrioles surrounded by an amorphous protein mass called the pericentriolar material …
The human microcephaly protein STIL interacts with CPAP and is required for procentriole formation
CJC Tang, SY Lin, WB Hsu, YN Lin, CT Wu… - The EMBO …, 2011 - embopress.org
Centriole duplication involves the growth of a procentriole next to the parental centriole.
Mutations in STIL and CPAP/CENPJ cause primary microcephaly (MCPH). Here, we show …
Mutations in STIL and CPAP/CENPJ cause primary microcephaly (MCPH). Here, we show …
Literature-curated protein interaction datasets
ME Cusick, H Yu, A Smolyar, K Venkatesan… - Nature …, 2009 - nature.com
High-quality datasets are needed to understand how global and local properties of protein-
protein interaction, or'interactome', networks relate to biological mechanisms, and to guide …
protein interaction, or'interactome', networks relate to biological mechanisms, and to guide …
SYNZIP Protein Interaction Toolbox: in Vitro and in Vivo Specifications of Heterospecific Coiled-Coil Interaction Domains
The synthetic biology toolkit contains a growing number of parts for regulating transcription
and translation, but very few that can be used to control protein association. Here we report …
and translation, but very few that can be used to control protein association. Here we report …
Empirically controlled mapping of the Caenorhabditis elegans protein-protein interactome network
To provide accurate biological hypotheses and elucidate global properties of cellular
networks, systematic identification of protein-protein interactions must meet high quality …
networks, systematic identification of protein-protein interactions must meet high quality …