▪ Abstract Heating therapies are increasingly used in cardiology, dermatology, gynecology,
neurosurgery, oncology, ophthalmology, orthopedics, and urology, among other medical …

Thermal denaturation-based methods are becoming increasingly used to characterize
protein stability and interactions. Recent technical advances have made these methods …

Thermal shift assays are used to study thermal stabilization of proteins upon ligand binding.
Such assays have been used extensively on purified proteins in the drug discovery industry …

The denaturation of immunoglobulin G was studied by different calorimetric methods and
circular dichroism spectroscopy. The thermogram of the immunoglobulin showed two main …

This article discusses several strategies for the use steady-state and time-resolved
fluorescence methods to monitor unfolding transitions in proteins. The assumptions and …

Recent research has provided an abundance of new information on membrane
biochemistry. Now more than ever, it is essential to update our current understanding of …

The effect of biotin binding on streptavidin (STV) structure and stability was studied using
differential scanning calorimetry, Fourier transform infrared spectroscopy (FT-IR), and …

The effect of biotin binding on the thermal stability of streptavidin (STV) and avidin (AVD)
was evaluated using differential scanning calorimetry. Biotin binding increases the midpoint …

Human manganese superoxide dismutase (MnSOD) is a homotetrameric enzyme which
protects mitochondria against oxygen-mediated free radical damage. Within each subunit …

Background In this case study, we analysed the properties of unfolded states and pathways
leading to complete denaturation of a multimeric chick pea β-galactosidase (Cp GAL), as …