Optical tweezers analysis of DNA–protein complexes
Cellular DNA is continuously engaged in a molecular dance with many accessory proteins.
These proteins are responsible for orchestrating the storage, maintenance, and transfer of …
These proteins are responsible for orchestrating the storage, maintenance, and transfer of …
When force met fluorescence: single-molecule manipulation and visualization of protein–DNA interactions
Myriad DNA-binding proteins undergo dynamic assembly, translocation, and conformational
changes while on DNA or alter the physical configuration of the DNA substrate to control its …
changes while on DNA or alter the physical configuration of the DNA substrate to control its …
The impact of DNA intercalators on DNA and DNA-processing enzymes elucidated through force-dependent binding kinetics
AS Biebricher, I Heller, RFH Roijmans… - Nature …, 2015 - nature.com
DNA intercalators are widely used as fluorescent probes to visualize DNA and DNA
transactions in vivo and in vitro. It is well known that they perturb DNA structure and stability …
transactions in vivo and in vitro. It is well known that they perturb DNA structure and stability …
Replication fork activation is enabled by a single-stranded DNA gate in CMG helicase
MR Wasserman, GD Schauer, ME O'Donnell, S Liu - Cell, 2019 - cell.com
The eukaryotic replicative helicase CMG is a closed ring around double-stranded (ds) DNA
at origins yet must transition to single-stranded (ss) DNA for helicase action. CMG must also …
at origins yet must transition to single-stranded (ss) DNA for helicase action. CMG must also …
Stabilization of DNA fork junctions by Smc5/6 complexes revealed by single-molecule imaging
NL Tanasie, P Gutierrez-Escribano, S Jaklin, L Aragon… - Cell reports, 2022 - cell.com
SMC complexes play key roles in genome maintenance, where they ensure efficient
genome replication and segregation. The SMC complex Smc5/6 is a crucial player in DNA …
genome replication and segregation. The SMC complex Smc5/6 is a crucial player in DNA …
Determination of single-molecule loading rate during mechanotransduction in cell adhesion
Cells connect with their environment through surface receptors and use physical tension in
receptor–ligand bonds for various cellular processes. Single-molecule techniques have …
receptor–ligand bonds for various cellular processes. Single-molecule techniques have …
Elastic properties and secondary structure formation of single-stranded DNA at monovalent and divalent salt conditions
A Bosco, J Camunas-Soler, F Ritort - Nucleic acids research, 2014 - academic.oup.com
Single-stranded DNA (ssDNA) plays a major role in several biological processes. It is
therefore of fundamental interest to understand how the elastic response and the formation …
therefore of fundamental interest to understand how the elastic response and the formation …
Single-molecule analysis reveals cooperative stimulation of Rad51 filament nucleation and growth by mediator proteins
Homologous recombination (HR) is an essential DNA double-strand break (DSB) repair
mechanism, which is frequently inactivated in cancer. During HR, RAD51 forms …
mechanism, which is frequently inactivated in cancer. During HR, RAD51 forms …
In vitro-reconstituted nucleoids can block mitochondrial DNA replication and transcription
G Farge, M Mehmedovic, M Baclayon… - Cell Reports, 2014 - cell.com
The mechanisms regulating the number of active copies of mtDNA are still unclear. A
mammalian cell typically contains 1,000–10,000 copies of mtDNA, which are packaged into …
mammalian cell typically contains 1,000–10,000 copies of mtDNA, which are packaged into …
Mfd dynamically regulates transcription via a release and catch-up mechanism
Summary The bacterial Mfd ATPase is increasingly recognized as a general transcription
factor that participates in the resolution of transcription conflicts with other …
factor that participates in the resolution of transcription conflicts with other …