Stability engineering of antibody single-chain Fv fragments
A WoÈrn, A PluÈckthun - Journal of molecular biology, 2001 - Elsevier
The application of single-chain Fv fragments (scFv) in medicine and biotechnology places
great demands on their stability. Only recently has attention been given to the production of …
great demands on their stability. Only recently has attention been given to the production of …
Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering
S Ewert, A Honegger, A Plückthun - Methods, 2004 - Elsevier
By combining the knowledge gained from an analysis of the biophysical properties of natural
antibody variable domains, the effects of mutations obtained in directed evolution …
antibody variable domains, the effects of mutations obtained in directed evolution …
Expression of an antibody fragment at high levels in the bacterial cytoplasm
P Martineau, P Jones, G Winter - Journal of molecular biology, 1998 - Elsevier
Recombinant antibody fragments expressed in the cytoplasm of cells have considerable
practical potential. However in the reducing environment of the cytoplasm, the intradomain …
practical potential. However in the reducing environment of the cytoplasm, the intradomain …
Influence of amino acid substitutions related to inherited human prion diseases on the thermodynamic stability of the cellular prion protein
S Liemann, R Glockshuber - Biochemistry, 1999 - ACS Publications
Transmissible spongiform encephalopathies (TSEs) are caused by a unique infectious
agent which appears to be identical with PrPSc, an oligomeric, misfolded isoform of the …
agent which appears to be identical with PrPSc, an oligomeric, misfolded isoform of the …
Antibody scFv fragments without disulfide bonds, made by molecular evolution
K Proba, A WoÈrn, A Honegger… - Journal of molecular …, 1998 - Elsevier
We generated stable and functional cysteine-free antibody single-chain fragments (scFv)
lacking the conserved disulfide bonds in both VH and VL. This was achieved by molecular …
lacking the conserved disulfide bonds in both VH and VL. This was achieved by molecular …
Structural determinants in the sequences of immunoglobulin variable domain
C Chothia, I Gelfand, A Kister - Journal of molecular biology, 1998 - Elsevier
To determine the general relation between the sequence and structure of variable domains
of immunoglobulins we have carried out an analysis of their atomic structures, some 5300 …
of immunoglobulins we have carried out an analysis of their atomic structures, some 5300 …
Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies
H Bach, Y Mazor, S Shaky, A Shoham-Lev… - Journal of molecular …, 2001 - Elsevier
Recombinant single-chain antibodies (scFvs) that are expressed in the cytoplasm of cells
are of considerable biotechnological and therapeutic potential. However, the reducing …
are of considerable biotechnological and therapeutic potential. However, the reducing …
Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent …
K Tsumoto, K Shinoki, H Kondo, M Uchikawa… - Journal of …, 1998 - Elsevier
An improved and efficient refolding system for a single-chain antibody fragment (scFv) from
inclusion bodies expressed in Escherichia coli was developed. Stepwise removal of …
inclusion bodies expressed in Escherichia coli was developed. Stepwise removal of …
How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system: Spectroscopic evidence for highly efficient refolding of a single …
M Umetsu, K Tsumoto, M Hara, K Ashish… - Journal of Biological …, 2003 - ASBMB
The gradual removal of the denaturing reagent guanidine HCl (GdnHCl) using stepwise
dialysis with the introduction of an oxidizing reagent and l-arginine resulted in the highly …
dialysis with the introduction of an oxidizing reagent and l-arginine resulted in the highly …
The selection of intracellular antibodies
A Cattaneo, S Biocca - Trends in biotechnology, 1999 - cell.com
The intracellular expression of antibodies in mammalian cells is a strategy to inhibit the in
vivo function of selected molecules but is limited by the unpredictable behaviour of …
vivo function of selected molecules but is limited by the unpredictable behaviour of …