Since the first administration of insulin to a person with diabetes in 1922, scientific
contributions from academia and industry have improved insulin therapy and access. The …

Saturation suppressor mutagenesis was used to generate thermostable mutants of the
SARS-CoV-2 spike receptor-binding domain (RBD). A triple mutant with an increase in …

In the realm of enzyme engineering, the dual enhancement of thermostability and activity
remains a challenge. Herein, we employed a computer-aided approach integrating folding …

Most amino acid substitutions in a protein either lead to partial loss-of-function or are near
neutral. Several studies have shown the existence of second-site mutations that can rescue …

The high-degree polymerization of isomaltooligosaccharide (IMO) not only effectively
promotes the growth and reproduction of Bifidobacterium in the human body but also …

While many computational methods accurately predict destabilizing mutations, identifying
stabilizing mutations has remained a challenge, because of their relative rarity. We tested …

Evolutionary engineering involves repeated mutations and screening and is widely used to
modify protein functions. However, it is important to diversify evolutionary pathways to …

The Mycobacterium tuberculosis genome harbours nine toxin-antitoxin (TA) systems of the
mazEF family. These consist of two proteins, a toxin and an antitoxin, encoded in an operon …

Protein stability is a requisite for most biotechnological and medical applications of proteins.
As natural proteins tend to suffer from a low conformational stability ex vivo, great efforts …

Congenital anomalies of the kidney and urinary tract (CAKUT) is the leading cause of
chronic kidney disease in the first three decades of life. Until now, more than 180 monogenic …